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lncRNA ANRIL accelerates wound healing in diabetic foot ulcers via modulating HIF1A/VEGFA signaling through interacting with FUS
Background Diabetic foot ulcer (DFU) is a frequently diagnosed complication of diabetes, and remains a heathcare burden worldwide. However, the pathogenesis of DFU is still largely unclear. The objective of this study is to delineate the function and underlying mechanism of lncRNA antisense non codi...
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Published in: | The journal of gene medicine 2023-02, Vol.25 (2), p.e3462-n/a |
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Main Authors: | , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Background
Diabetic foot ulcer (DFU) is a frequently diagnosed complication of diabetes, and remains a heathcare burden worldwide. However, the pathogenesis of DFU is still largely unclear. The objective of this study is to delineate the function and underlying mechanism of lncRNA antisense non coding RNA in the INK4 locus (ANRIL) in endothelial progenitor cells (EPCs) and DFU mice.
Methods
The DFU mouse model was established, and EPCs were subjected to high glucose (HG) treatment to mimic diabetes. qRT‐PCR or western blot was employed to detected the expression of ANRIL, HIF1A, FUS and VEGFA. CCK‐8 and Annexin V/PI staining were used to monitor cell proliferation and apoptosis. Wound healing, Transwell invasion and tube formation assays were conducted to assess cell migration, invasion and angiogenesis, respectively. The association between ANRIL and FUS was verified by RNA pull‐down and RIP assays. Luciferase and ChIP assays were employed to investigate HIF1A‐mediated transcriptional regulation of VEGFA and ANRIL. The histological alterations of DFU wound healing were observed by H&E and Masson staining.
Results
ANRIL was downregulated in peripheral blood samples of DFU patients, DFU mice and HG‐treated EPCs. Mechanistically, ANRIL regulated HIFA mRNA stability via recruiting FUS. VEGFA and ANRIL were transcriptionally regulated by HIF1A. Functional experiments revealed that HG suppressed EPC proliferation, migration, invasion and tube formation, but promoted apoptosis via ANRIL/HIF1A axis. ANRIL accelerated DFU wound healing via modulating HIF1A expression in vivo.
Conclusion
ANRIL accelerated wound healing in DFU via modulating HIF1A/VEGFA signaling in a FUS‐dependent manner.
LncRNA ANRIL accelerated wound healing in diabetic foot ulcer (DFU) via modulating HIF1A/VEGFA signaling. Mechanistically, ANRIL enhanced HIF1A mRNA stability via recruiting FUS. VEGFA and ANRIL were transcriptionally regulated by HIF1A. |
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ISSN: | 1099-498X 1521-2254 |
DOI: | 10.1002/jgm.3462 |