Fabrication of nitrogen-doped graphene quantum dots based fluorescent probe and its application for simultaneous, sensitive and selective detection of umami amino acids

•The N-GQDs were synthesized via a one-step green hydrothermal method conveniently.•The N-GQDs showed unique fluorescent luminescence properties.•A fluorescent probe was constructed for simultaneous detection of l-Glu and l-Asp.•Excellent selectivity, stability, reproducibility and applicability wer...

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Bibliographic Details
Published in:Food chemistry 2023-03, Vol.404, p.134509-134509, Article 134509
Main Authors: Han, Tingting, Huang, Yang, Gao, Tianyi, Xia, Chunyue, Sun, Chong, Xu, Weimin, Wang, Daoying
Format: Article
Language:English
Subjects:
Fluorescent probe
l-aspartic acid
l-glutamic acid
Nitrogen-doped graphene quantum dots
Simultaneous detection
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Summary:•The N-GQDs were synthesized via a one-step green hydrothermal method conveniently.•The N-GQDs showed unique fluorescent luminescence properties.•A fluorescent probe was constructed for simultaneous detection of l-Glu and l-Asp.•Excellent selectivity, stability, reproducibility and applicability were presented. In this work, nitrogen-doped graphene quantum dots (N-GQDs) were synthesized by an efficient one-step hydrothermal using hydrated citric acid and urea as the carbon and nitrogen sources, respectively. The fluorescent quantum yield of the N-GQDs was up to 88.20 %, and the fluorescence lifetime was 12.29 ns. Based on the excellent fluorescence property, water-soluble and low cytotoxicity of N-GQDs, a novel fluorescent probe was fabricated and used for simultaneous detection of l-glutamic acid (l-Glu) and l-aspartic acid (l-Asp). Herein, Al3+ was used as a chelant agent for l-Glu shielding. The sensitive response of the probe towards l-Glu and l-Asp presented a wide concentration range with a low detection limit (S/N = 3). The fluorescent probe showed good selectivity, high stability and acceptable reproducibility in the application of l-Glu/l-Asp simultaneous detection in chicken soup. Meanwhile, the detection results of the fluorescent probe were correlated well with those obtained by amino acid analyzer.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2022.134509