Immobilization of PETase enzymes on magnetic iron oxide nanoparticles for the decomposition of microplastic PET

Polyethylene terephthalate (PET) is responsible for a large amount of environmental contamination with microplastics. Based on its high affinity, the PET degrading enzyme PETase can be immobilized on superparamagnetic iron oxide nanoparticles through a His-tag. The His-tag increases enzyme stability...

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Bibliographic Details
Published in:Nanoscale advances 2021-07, Vol.3 (15), p.4395-4399
Main Authors: Schwaminger, Sebastian P, Fehn, Stefan, Steegmüller, Tobias, Rauwolf, Stefan, Löwe, Hannes, Pflüger-Grau, Katharina, Berensmeier, Sonja
Format: Article
Language:English
Subjects:
Chemistry
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Summary:Polyethylene terephthalate (PET) is responsible for a large amount of environmental contamination with microplastics. Based on its high affinity, the PET degrading enzyme PETase can be immobilized on superparamagnetic iron oxide nanoparticles through a His-tag. The His-tag increases enzyme stability, and allows magnetic separation for recovery. Multiple recycling steps are possible and microplastic particles can be decomposed depending on the PET's crystallinity. The separation or decomposition of PET allows for a sustainable way to remove microplastic from water. The enzyme PETase can be attached to superparamagnetic iron oxide nanoparticles through a His-tag facilitating a high enzymatic activity for the degradation of PET microplastic particles.
ISSN:2516-0230
2516-0230
DOI:10.1039/d1na00243k