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Quantitative histology as a diagnostic tool for celiac disease in children and adolescents
To measure the villous height, the crypt depth, and the number of intraepithelial lymphocytes/100 enterocytes of the small intestinal mucosa of children and adolescents with celiac disease; and to classify these findings according to Q- Marsh and Q-histology scales. Retrospective study of a database...
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| Published in: | Annals of diagnostic pathology 2022-12, Vol.61, p.152031-152031, Article 152031 |
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| creator | Vargas, Mateus M. Artigiani Neto, Ricardo Sdepanian, Vera L. |
| description | To measure the villous height, the crypt depth, and the number of intraepithelial lymphocytes/100 enterocytes of the small intestinal mucosa of children and adolescents with celiac disease; and to classify these findings according to Q- Marsh and Q-histology scales.
Retrospective study of a database from the Department of Pathology of biopsies from the second portion of the duodenum of pediatric patients. According to the histological report, three groups were established: celiac disease at diagnosis (n = 50), controls (n = 26), giardiasis (n = 10). In each biopsy, software (cellSens and Image J) evaluated 5 villous heights, 5 crypt depth and the number of intraepithelial lymphocytes/100 enterocytes.
The celiac group had the lowest mean villous height (197.83 μm) of all three groups (control = 477.70 μm; giardiasis = 397.04 μm. The celiac group's villous:crypt ratio (0.78) was significantly lower than the control group (1.89). The number of intraepithelial lymphocytes ≥25 was exclusive to the celiac group, with a sensitivity and specificity of 100 %. Only celiac patients were included in types 2 and 3 of the Q-histology classification.
Celiac disease patients showed shorter villous height than other groups, and the number of intraepithelial lymphocytes ≥25 was the best parameter to differentiate celiac from controls and giardiasis groups. Intraepithelial lymphocytes ≥25/100 enterocytes associated with any degree of villous atrophy, the classic Marsh 3 type, set the histological parameters of celiac disease. Quantitative histology is a valuable tool for diagnosing celiac disease, enabling histological changes in a short time, and the Q-histology scale appears to be more suitable than the Q-Marsh scale.
•Quantitative histology helps discriminate celiac disease from other conditions.•Q-histology is the best morphometric scale for pediatrics.•Intraepithelial lymphocytes count ≥25 is the best parameter for diagnosis.•Intraepithelial lymphocytes ≥25 and any degree of villous atrophy set diagnosis. |
| doi_str_mv | 10.1016/j.anndiagpath.2022.152031 |
| format | article |
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Retrospective study of a database from the Department of Pathology of biopsies from the second portion of the duodenum of pediatric patients. According to the histological report, three groups were established: celiac disease at diagnosis (n = 50), controls (n = 26), giardiasis (n = 10). In each biopsy, software (cellSens and Image J) evaluated 5 villous heights, 5 crypt depth and the number of intraepithelial lymphocytes/100 enterocytes.
The celiac group had the lowest mean villous height (197.83 μm) of all three groups (control = 477.70 μm; giardiasis = 397.04 μm. The celiac group's villous:crypt ratio (0.78) was significantly lower than the control group (1.89). The number of intraepithelial lymphocytes ≥25 was exclusive to the celiac group, with a sensitivity and specificity of 100 %. Only celiac patients were included in types 2 and 3 of the Q-histology classification.
Celiac disease patients showed shorter villous height than other groups, and the number of intraepithelial lymphocytes ≥25 was the best parameter to differentiate celiac from controls and giardiasis groups. Intraepithelial lymphocytes ≥25/100 enterocytes associated with any degree of villous atrophy, the classic Marsh 3 type, set the histological parameters of celiac disease. Quantitative histology is a valuable tool for diagnosing celiac disease, enabling histological changes in a short time, and the Q-histology scale appears to be more suitable than the Q-Marsh scale.
•Quantitative histology helps discriminate celiac disease from other conditions.•Q-histology is the best morphometric scale for pediatrics.•Intraepithelial lymphocytes count ≥25 is the best parameter for diagnosis.•Intraepithelial lymphocytes ≥25 and any degree of villous atrophy set diagnosis.</description><identifier>ISSN: 1092-9134</identifier><identifier>EISSN: 1532-8198</identifier><identifier>DOI: 10.1016/j.anndiagpath.2022.152031</identifier><language>eng</language><publisher>Elsevier Inc</publisher><subject>Adolescents ; Celiac disease/diagnosis ; Children ; Giardiasis ; Histology ; Intestinal Mucosa</subject><ispartof>Annals of diagnostic pathology, 2022-12, Vol.61, p.152031-152031, Article 152031</ispartof><rights>2022 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c354t-154fbba53422f2bc1862e3324ef6319ae2446d735f83c92f39d0843b3cb1b4893</citedby><cites>FETCH-LOGICAL-c354t-154fbba53422f2bc1862e3324ef6319ae2446d735f83c92f39d0843b3cb1b4893</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids></links><search><creatorcontrib>Vargas, Mateus M.</creatorcontrib><creatorcontrib>Artigiani Neto, Ricardo</creatorcontrib><creatorcontrib>Sdepanian, Vera L.</creatorcontrib><title>Quantitative histology as a diagnostic tool for celiac disease in children and adolescents</title><title>Annals of diagnostic pathology</title><description>To measure the villous height, the crypt depth, and the number of intraepithelial lymphocytes/100 enterocytes of the small intestinal mucosa of children and adolescents with celiac disease; and to classify these findings according to Q- Marsh and Q-histology scales.
Retrospective study of a database from the Department of Pathology of biopsies from the second portion of the duodenum of pediatric patients. According to the histological report, three groups were established: celiac disease at diagnosis (n = 50), controls (n = 26), giardiasis (n = 10). In each biopsy, software (cellSens and Image J) evaluated 5 villous heights, 5 crypt depth and the number of intraepithelial lymphocytes/100 enterocytes.
The celiac group had the lowest mean villous height (197.83 μm) of all three groups (control = 477.70 μm; giardiasis = 397.04 μm. The celiac group's villous:crypt ratio (0.78) was significantly lower than the control group (1.89). The number of intraepithelial lymphocytes ≥25 was exclusive to the celiac group, with a sensitivity and specificity of 100 %. Only celiac patients were included in types 2 and 3 of the Q-histology classification.
Celiac disease patients showed shorter villous height than other groups, and the number of intraepithelial lymphocytes ≥25 was the best parameter to differentiate celiac from controls and giardiasis groups. Intraepithelial lymphocytes ≥25/100 enterocytes associated with any degree of villous atrophy, the classic Marsh 3 type, set the histological parameters of celiac disease. Quantitative histology is a valuable tool for diagnosing celiac disease, enabling histological changes in a short time, and the Q-histology scale appears to be more suitable than the Q-Marsh scale.
•Quantitative histology helps discriminate celiac disease from other conditions.•Q-histology is the best morphometric scale for pediatrics.•Intraepithelial lymphocytes count ≥25 is the best parameter for diagnosis.•Intraepithelial lymphocytes ≥25 and any degree of villous atrophy set diagnosis.</description><subject>Adolescents</subject><subject>Celiac disease/diagnosis</subject><subject>Children</subject><subject>Giardiasis</subject><subject>Histology</subject><subject>Intestinal Mucosa</subject><issn>1092-9134</issn><issn>1532-8198</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqNkEtLAzEUhYMoWKv_Ie7czJjnzGQpxRcURNCNm5DJ3GlTpklN0kL_vVPGhUtX98I953DPh9AtJSUltLrflMb7zpnVzuR1yQhjJZWMcHqGZlRyVjRUNefjThQrFOXiEl2ltCGEUiHrGfp63xufXTbZHQCvXcphCKsjNgkbfMr1IWVncQ5hwH2I2MLgjB1PCUwC7Dy2azd0ETw2vsOmCwMkCz6na3TRmyHBze-co8-nx4_FS7F8e35dPCwLy6XIBZWib1sjuWCsZ62lTcWAcyagrzhVBpgQVVdz2TfcKtZz1ZFG8JbblraiUXyO7qbcXQzfe0hZb934wTAYD2GfNKuJqrlSUo5SNUltDClF6PUuuq2JR02JPvHUG_2Hpz7x1BPP0buYvDB2OTiIOlkH3kLnItisu-D-kfID9HCEzw</recordid><startdate>202212</startdate><enddate>202212</enddate><creator>Vargas, Mateus M.</creator><creator>Artigiani Neto, Ricardo</creator><creator>Sdepanian, Vera L.</creator><general>Elsevier Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202212</creationdate><title>Quantitative histology as a diagnostic tool for celiac disease in children and adolescents</title><author>Vargas, Mateus M. ; Artigiani Neto, Ricardo ; Sdepanian, Vera L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c354t-154fbba53422f2bc1862e3324ef6319ae2446d735f83c92f39d0843b3cb1b4893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Adolescents</topic><topic>Celiac disease/diagnosis</topic><topic>Children</topic><topic>Giardiasis</topic><topic>Histology</topic><topic>Intestinal Mucosa</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vargas, Mateus M.</creatorcontrib><creatorcontrib>Artigiani Neto, Ricardo</creatorcontrib><creatorcontrib>Sdepanian, Vera L.</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of diagnostic pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vargas, Mateus M.</au><au>Artigiani Neto, Ricardo</au><au>Sdepanian, Vera L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative histology as a diagnostic tool for celiac disease in children and adolescents</atitle><jtitle>Annals of diagnostic pathology</jtitle><date>2022-12</date><risdate>2022</risdate><volume>61</volume><spage>152031</spage><epage>152031</epage><pages>152031-152031</pages><artnum>152031</artnum><issn>1092-9134</issn><eissn>1532-8198</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>To measure the villous height, the crypt depth, and the number of intraepithelial lymphocytes/100 enterocytes of the small intestinal mucosa of children and adolescents with celiac disease; and to classify these findings according to Q- Marsh and Q-histology scales.
Retrospective study of a database from the Department of Pathology of biopsies from the second portion of the duodenum of pediatric patients. According to the histological report, three groups were established: celiac disease at diagnosis (n = 50), controls (n = 26), giardiasis (n = 10). In each biopsy, software (cellSens and Image J) evaluated 5 villous heights, 5 crypt depth and the number of intraepithelial lymphocytes/100 enterocytes.
The celiac group had the lowest mean villous height (197.83 μm) of all three groups (control = 477.70 μm; giardiasis = 397.04 μm. The celiac group's villous:crypt ratio (0.78) was significantly lower than the control group (1.89). The number of intraepithelial lymphocytes ≥25 was exclusive to the celiac group, with a sensitivity and specificity of 100 %. Only celiac patients were included in types 2 and 3 of the Q-histology classification.
Celiac disease patients showed shorter villous height than other groups, and the number of intraepithelial lymphocytes ≥25 was the best parameter to differentiate celiac from controls and giardiasis groups. Intraepithelial lymphocytes ≥25/100 enterocytes associated with any degree of villous atrophy, the classic Marsh 3 type, set the histological parameters of celiac disease. Quantitative histology is a valuable tool for diagnosing celiac disease, enabling histological changes in a short time, and the Q-histology scale appears to be more suitable than the Q-Marsh scale.
•Quantitative histology helps discriminate celiac disease from other conditions.•Q-histology is the best morphometric scale for pediatrics.•Intraepithelial lymphocytes count ≥25 is the best parameter for diagnosis.•Intraepithelial lymphocytes ≥25 and any degree of villous atrophy set diagnosis.</abstract><pub>Elsevier Inc</pub><doi>10.1016/j.anndiagpath.2022.152031</doi><tpages>1</tpages></addata></record> |
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| subjects | Adolescents Celiac disease/diagnosis Children Giardiasis Histology Intestinal Mucosa |
| title | Quantitative histology as a diagnostic tool for celiac disease in children and adolescents |
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