Supplementation of porcine in vitro maturation medium with FGF2, LIF, and IGF1 enhances cytoplasmic maturation in prepubertal gilts oocytes and improves embryo quality

Summary In porcine in vitro production (IVP) systems, the use of oocytes derived from prepubertal gilts, whilst being commercially attractive, remains challenging due to their poor developmental competence following in vitro maturation (IVM). Follicular fluid contains important growth factors and pl...

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Bibliographic Details
Published in:Zygote (Cambridge) 2022-12, Vol.30 (6), p.801-808
Main Authors: Serrano Albal, María, Silvestri, Giuseppe, Kiazim, Lucas G., Vining, Lucy M., Zak, Louisa J., Walling, Grant A., Haigh, Alexandra M., Harvey, Simon C., Harvey, Katie E., Griffin, Darren K.
Format: Article
Language:English
Subjects:
Abattoirs
Amino acids
Biosecurity
Cytokines
Embryos
Fibroblast growth factor 2
Follicular fluid
Gametocytes
Growth factors
Hogs
Hypotheses
In vitro fertilization
Insulin
Insulin-like growth factor I
Leukemia
Leukemia inhibitory factor
Maturation
Meiosis
Oocytes
Ovaries
Sodium
Sperm
Supplements
Synergistic effect
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Summary:Summary In porcine in vitro production (IVP) systems, the use of oocytes derived from prepubertal gilts, whilst being commercially attractive, remains challenging due to their poor developmental competence following in vitro maturation (IVM). Follicular fluid contains important growth factors and plays a key role during oocyte maturation; therefore, it is a common supplementation for porcine IVM medium. However, follicular fluid contains many poorly characterized components, is batch variable, and its use raises biosecurity concerns. In an effort to design a defined IVM system, growth factors such as cytokines have been previously tested. These include leukaemia inhibitory factor (LIF), fibroblast growth factor 2 (FGF2), and insulin-like growth factor 1 (IGF1), the combination of which is termed ‘FLI’. Here, using abattoir-derived oocytes in a well established porcine IVP system, we compared follicular fluid and FLI supplementation during both IVM and embryo culture to test the hypothesis that FLI can substitute for follicular fluid without compromising oocyte nuclear and cytoplasmic maturation. We demonstrate that in oocytes derived from prepubertal gilts, FLI supplementation enhances oocyte meiotic maturation and has a positive effect on the quality and developmental competence of embryos. Moreover, for the first time, we studied the effects of follicular fluid and FLI combined showing no synergistic effects.
ISSN:0967-1994
1469-8730
DOI:10.1017/S0967199422000284