Ameliorative effect of bone marrow-derived mesenchymal stem cells on burn-induced hepatic and metabolic derangements in rats

The current study aims to investigate the therapeutic potential of bone marrow-derived mesenchymal stem cells (MSCs) as a solo therapy in ameliorating both skin lesions and liver injury induced by cutaneous severe burn injury (SBI) in rats. In anesthetized male adult Wistar albino rats, 30 % total b...

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Bibliographic Details
Published in:Life sciences (1973) 2022-10, Vol.307, p.120891-120891, Article 120891
Main Authors: Shibl, Nourhan G., Fikry, Ebtehal Mohammad, Mansour, Hanaa A., Alsemeh, Amira Ebrahim, Abdel-Ghany, Rasha H., El-Sayed, Shaimaa S.
Format: Article
Language:English
Subjects:
Hyperglycemia
Hyperinflammation
Insulin receptor substrate-1
Liver
Mesenchymal stem cells
Severe burn injury
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Summary:The current study aims to investigate the therapeutic potential of bone marrow-derived mesenchymal stem cells (MSCs) as a solo therapy in ameliorating both skin lesions and liver injury induced by cutaneous severe burn injury (SBI) in rats. In anesthetized male adult Wistar albino rats, 30 % total burn surface area and established hepatic injury was achieved via direct contact of each experimental animal's dorsum with heated metal rod (100 °C) for 10 s. On the next day following burn, human MSCs or mouse MSCs was administered locally around the burn site and intraperitonially (0.5 × 106 cells/rat for each route) and outcomes were investigated at 4 and 14 days following burn induction. Both types of MSCs significantly improved skin and liver histology, decreased liver enzymes, and ameliorated oxidative stress in hepatocytes of SBI-rats. Further, SBI-induced rises in hepatic apoptotic marker (caspase-3, Bax) and serum inflammatory markers (TNF-α, IL-1β, and IL-6) were reduced following either human or mouse MSC administration. In addition, MSCs augmented insulin receptor substrate-1, phosphorylated protein kinase-B (phospho-Akt), while alleviating serum glucose levels in SBI-rats. These previous effects persisted even at the 14-day time point. Following single administration, bone marrow-derived MSCs is capable of counteracting SBI-induced skin lesions as well as related hepatic complications, specifically via mitigating postburn hyperglycemia and hyperinflammation. [Display omitted] •Severe burn predisposes to hepatic injury.•Altered metabolism with insulin resistance is an outcome in severe burn injury.•Mesenchymal stem cells alleviated hepatic injury associated with severe burn.•Mesenchymal stem cells attenuated hepatic oxidative stress and apoptosis in severe burn.•Mesenchymal stem cells mitigated insulin resistance post severe burn.
ISSN:0024-3205
1879-0631
DOI:10.1016/j.lfs.2022.120891