Electrochemical determination of emodin in acidic media by high-performance liquid chromatography and its application to Polygoni Multiflori Radix samples

Electrochemical reduction of emodin under acidic media occurs at a less negative potential when compared with that under neutral media. When emodin is electrochemically detected at a less negative potential, a decrease in background noise and improvement in specificity benefit the development of hig...

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Bibliographic Details
Published in:Analytical sciences 2022-11, Vol.38 (11), p.1449-1454
Main Authors: Shiozawa, Aya, Kojima, Yusuke, Kotani, Akira, Machida, Koichi, Yamamoto, Kazuhiro, Hakamata, Hideki
Format: Article
Language:English
Subjects:
Analytical Chemistry
Chemistry
Chemistry and Materials Science
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Summary:Electrochemical reduction of emodin under acidic media occurs at a less negative potential when compared with that under neutral media. When emodin is electrochemically detected at a less negative potential, a decrease in background noise and improvement in specificity benefit the development of high-performance liquid chromatography with electrochemical detection (HPLC-ECD) for its determination. HPLC-ECD was performed using an octadecyl silica column, acetonitrile–water (60:40, v/v) containing 5 mmol L −1 hydrochloric acid and 10 mmol L −1 lithium perchlorate, as a mobile phase, and an applied potential at − 0.4 V vs. Ag/AgCl. Under these optimal HPLC-ECD conditions, the detection limit (signal-to-noise ratio, S/N = 3) of emodin was 0.61 μg L −1 . When this HPLC-ECD system was applied to the determination of emodin in Polygoni Multiflori Radix (PMR) samples, other peaks did not appear close to the emodin peak on a chromatogram. The emodin contents in PMR samples were determined with relative standard deviations (RSDs, n  = 6) of less than 3.9%, and their recoveries ranged from 92 to 106%. We have shown that our HPLC-ECD system performed an accurate, precise, and specific determination of emodin in PMR samples. Graphical abstract
ISSN:0910-6340
1348-2246
DOI:10.1007/s44211-022-00177-5