A highly sensitive NIR fluorescence probe for hypoxia imaging in cells and ulcerative colitis

Ulcerative colitis, a kind of inflammatory bowel disease (IBD), is caused by dysregulated immune response of intestinal bacteria. This chronic disorder can lead to a deficiency of O2 (hypoxia) in the colon microenvironment. Nitroreductase (NTR) is a highly expressed endogenous enzyme under hypoxia,...

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Bibliographic Details
Published in:Talanta (Oxford) 2023-01, Vol.252, p.123834-123834, Article 123834
Main Authors: Wang, Sheng, Zhang, Xiao-Fan, Wang, He-Song, Liu, Jing, Shen, Shi-Li, Cao, Xiao-Qun
Format: Article
Language:English
Subjects:
Hypoxia imaging
Near-infrared fluorescence
Nitroreductase
Tumor
Ulcerative colitis
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Summary:Ulcerative colitis, a kind of inflammatory bowel disease (IBD), is caused by dysregulated immune response of intestinal bacteria. This chronic disorder can lead to a deficiency of O2 (hypoxia) in the colon microenvironment. Nitroreductase (NTR) is a highly expressed endogenous enzyme under hypoxia, so the detection of NTR can provide diagnostic information about ulcerative colitis. Herein, an ultrasensitive NTR-triggered fluorescence probe (WS-1-NO2) is developed for hypoxia imaging in ulcerative colitis. The probe shows a significant fluorescence enhancement (45-fold) after reacting with NTR, with an extremely low detection limit of 0.096 ng/mL. Furthermore, we apply it for fluorescence imaging of hypoxia in living cells, tumors and dextran sulphate sodium (DSS)-induced ulcerative colitis mouse models. We believe that the probe may be investigated as an effective potential tool for gaining insight into the hypoxia-relevant diseases, such as cancer and ulcerative colitis. [Display omitted] •An ultrasensitive NIR fluorescence probe for NTR is developed with a low detection limit of 0.096 ng/mL.•The probe can be used to detect hypoxia in living cells.•The probe is applied for the visualization of NTR in hypoxic tumors.•In vivo accurate imaging of hypoxia is realized in ulcerative colitis mouse model.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2022.123834