Tumor necrosis factor alpha (TNF-α) blockage reduces acute inflammation and delayed wound healing in oral ulcer of rats

Oral traumatic ulcers (OTU) are common in dental routine, and the control of proinflammatory cytokines, such as the tumor necrosis factor-alpha (TNF-α), may interfere with OTU repair. Our aim was to evaluate the role of TNF-α in the healing process of OTU in rats. Wistar male rats were divided into...

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Bibliographic Details
Published in:Inflammopharmacology 2022-10, Vol.30 (5), p.1781-1798
Main Authors: Freitas, Milena Oliveira, Fonseca, Alline Pereira Rodrigues, de Aguiar, Maria Thaynara, Dias, Camila Costa, Avelar, Rafael Linard, Sousa, Fabrício Bitu, Alves, Ana Paula Negreiros Nunes, de Barros Silva, Paulo Goberlânio
Format: Article
Language:English
Subjects:
Allergology
Biomedical and Life Sciences
Biomedicine
Dermatology
Gastroenterology
Immunology
Original Article
Pharmacology/Toxicology
Rheumatology
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Summary:Oral traumatic ulcers (OTU) are common in dental routine, and the control of proinflammatory cytokines, such as the tumor necrosis factor-alpha (TNF-α), may interfere with OTU repair. Our aim was to evaluate the role of TNF-α in the healing process of OTU in rats. Wistar male rats were divided into six groups: a control-group (treated with 0.1 mL/kg of saline) and five groups treated with anti-TNF-α infliximab (INF) at 1, 3, 5, 7, and 10 mg/kg immediately before OTU production. The animals were weighed (day 0) and euthanized on days 1, 3, 7, 14 and 21 after ulceration. The ulcers were clinically measured, and the mucosa samples were histologically (scores 0–4), histochemically (collagen assay (pircrosirius)), histomorphometrically (cell counting), and immunohistochemically (TNF-α, α-smooth-muscle-actin (α-SMA), monocyte-chemoattractive-protein-1 (MCP-1), interleukin-8 (IL-8), and fibroblast-growth-factor (FGF)) analyzed. The Evans blue assay was used to measure the vascular permeability. ANOVA-1–2-way/Bonferroni, Kruskal–Wallis/Dunn, and correlation analyses were performed (GraphPad Prism 5.0, p  
ISSN:0925-4692
1568-5608
DOI:10.1007/s10787-022-01046-3