Hypothetical bromodomain-containing protein 5 is required for the growth of Toxoplasma gondii

Bromodomain (BRD) is a highly conserved structural module domain, found in various proteins, including chromatin-related proteins, nucleus acetyltransferases, and transcription-associated proteins. Toxoplasma gondii, a zoonotic protozoan, encodes at least 12 predicted BRD-containing proteins (BDPs)....

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Published in:Veterinary parasitology 2022-09, Vol.309, p.109767-109767, Article 109767
Main Authors: Zhang, Ying, Cheng, Long, Qiu, Hongyu, Sun, Tiancong, Deng, Ruipeng, Gong, Haiyan, Mi, Rongsheng, Huang, Yan, Wang, Chunren, Chen, Zhaoguo
Format: Article
Language:English
Subjects:
Bromodomain-containing protein 5
Conditional ablation
Localization
Proliferation
Toxoplasma gondii
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Summary:Bromodomain (BRD) is a highly conserved structural module domain, found in various proteins, including chromatin-related proteins, nucleus acetyltransferases, and transcription-associated proteins. Toxoplasma gondii, a zoonotic protozoan, encodes at least 12 predicted BRD-containing proteins (BDPs). Here, we investigated the subcellular location and regulatory role of a hypothetical protein BDP that we named TgBDP5. The BRD of TgBDP5 did not contain the conserved Asn and Tyr residues required for acetyl-lysine recognition. TgBDP5 localized in the nucleus of the parasite and remained unchanged during parasite replication. Conditional ablation of TgBDP5 through an auxin-inducible degron-based knockdown strategy caused a growth defect in parasite replication. Depletion of TgBDP5 led to changes in the expression level of 179 genes, suggesting it as an important target for drugs acting against T. gondii. •TgBDP5 is required for the replication in the lytic cycle of T. gondii.•179 genes were regulated in the TgBDP5 which depleted T. gondii strain.•The ablation of TgBDP5 is closely related to various KEGG pathways.•TgBDP5 localized in the nucleus and did not change during replication.
ISSN:0304-4017
1873-2550
DOI:10.1016/j.vetpar.2022.109767