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Streptomyces sp. BV410: Interspecies cross‐talk for staurosporine production
Aims Sequencing and genome analysis of two co‐isolated streptomycetes, named BV410‐1 and BV410‐10, and the effect of their co‐cultivation on the staurosporine production. Methods and Results Identification of two strains through genome sequencing and their separation using different growth media was...
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Published in: | Journal of applied microbiology 2022-10, Vol.133 (4), p.2560-2568 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Aims
Sequencing and genome analysis of two co‐isolated streptomycetes, named BV410‐1 and BV410‐10, and the effect of their co‐cultivation on the staurosporine production.
Methods and Results
Identification of two strains through genome sequencing and their separation using different growth media was conducted. Sequence analysis revealed that the genome of BV410‐1 was 9.5 Mb, whilst that of BV410‐10 was 7.1 Mb. AntiSMASH analysis identified 28 biosynthetic gene clusters (BGCs) from BV410‐1, including that responsible for staurosporine biosynthesis, whilst 20 BGCs were identified from BV410‐10. The addition of cell‐free supernatant from BV410‐10 monoculture to BV410‐1 fermentations improved the staurosporine yield from 8.35 mg L−1 up to 15.85 mg L−1, whilst BV410‐10 monoculture ethyl acetate extract did not have the same effect. Also, there was no improvement in staurosporine production when artificial mixed cultures were created using three different BV410‐1 and BV410‐10 spore ratios.
Conclusions
The growth of BV410‐10 was inhibited when the two strains were grown together on agar plates. Culture supernatants of BV410‐10 showed potential to stimulate staurosporine production in BV410‐1, but overall co‐cultivation attempts did not restore the previously reported yield of staurosporine produced by the original mixed isolate.
Significance and Impact of Study
This work confirmed complex relations between streptomycetes in soil that are difficult to recreate under the laboratory conditions. Also, mining of streptomycetes genomes that mainly produce known bioactive compounds could still be the fruitful approach in search for novel bioactive molecules. |
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ISSN: | 1364-5072 1365-2672 |
DOI: | 10.1111/jam.15726 |