Validation of a liquid chromatography coupled to tandem mass spectrometry method for simultaneous quantification of tryptophan and 10 key metabolites of the kynurenine pathway in plasma and urine: Application to a cohort of acute kidney injury patients

A sensitive and rapid liquid chromatography-tandem mass spectrometry (LC–MS/MS) method was developed and validated for the simultaneous determination of tryptophan (Trp) and ten metabolites of kynurenine pathway, including kynurenine (Kyn), 3-hydroxy-kynurenine (3-HK), kynurenic acid (KA), xanthuren...

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Bibliographic Details
Published in:Clinica chimica acta 2022-09, Vol.534, p.115-127
Main Authors: Nadour, Zahia, Simian, Christophe, Laprévote, Olivier, Loriot, Marie-Anne, Larabi, Islam Amine, Pallet, Nicolas
Format: Article
Language:English
Subjects:
Acute kidney injury
Kynurenic acid
Kynurenine pathway
Mass spectrometry
Picolinic acid
Quinolinic acid
Tryptophan
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Summary:A sensitive and rapid liquid chromatography-tandem mass spectrometry (LC–MS/MS) method was developed and validated for the simultaneous determination of tryptophan (Trp) and ten metabolites of kynurenine pathway, including kynurenine (Kyn), 3-hydroxy-kynurenine (3-HK), kynurenic acid (KA), xanthurenic acid (XA), 3-Hydroxy-anthranilic acid (3-HANA), quinolinic acid (QA), nicotinic acid mononucleotide (NaMN), picolinic acid (Pic), nicotinamide (NAM) and nicotinic acid (NA) in both plasma and urine. This LC-MS/MS method was used to predict the occurrence of acute kidney injury (AKI) in a cohort of patients with cardiac surgery under cardiopulmonary bypass (CPB). Urinary concentrations of Pic, as well as Pic to Trp and Pic to 3-HANA ratios were highly predictive of an AKI episode the week after CPB, indicating that Pic could be a predictive biomarker of AKI. Thus, monitoring the kynurenine pathway activity with this LC–MS/MS method is a clinically relevant tool to identify new biomarkers of kidney injury.
ISSN:0009-8981
1873-3492
DOI:10.1016/j.cca.2022.07.009