Expression analysis of gibberellin-regulated protein in peach by reverse transcription-quantitative PCR

ABSTRACT Gibberellin-regulated protein (GRP) is a fruit severe allergen. The amounts of GRP expression normalized against actin in peach were determined by reverse transcription-quantitative PCR (RT-qPCR). The results were consistent with those determined by enzyme-linked immunosorbent assay (ELISA)...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2022-09, Vol.86 (10), p.1459-1461
Main Authors: Kawai, Natsuki, Hirakawa, Yuki, Matsumoto, Shinya, Itai, Akihiro, Matsunaga, Kayoko, Narita, Hiroshi, Momma, Keiko
Format: Article
Language:English
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Summary:ABSTRACT Gibberellin-regulated protein (GRP) is a fruit severe allergen. The amounts of GRP expression normalized against actin in peach were determined by reverse transcription-quantitative PCR (RT-qPCR). The results were consistent with those determined by enzyme-linked immunosorbent assay (ELISA). The GRP expression was more evident in flesh than peel and increased rapidly in the maturing period. This approach is applicable to estimate the amount of GRP in other plants.
ISSN:1347-6947
1347-6947
DOI:10.1093/bbb/zbac123