Inverse correlation between the expression of AMPK/SIRT1 and NAMPT in psoriatic skin: A pilot study

Epidermal hyperplasia and the involvement of immune cells characterize the clinical picture of psoriasis. Among the several factors involved, attention has been focused on sirtuin 1 (SIRT1) - a deacetylase endowed with a variety of functions including the control of metabolic and inflammatory proces...

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Bibliographic Details
Published in:	Advances in medical sciences 2022-09, Vol.67 (2), p.262-268
Main Authors:	D'Amico, Fabio, Costantino, Giuseppe, Salvatorelli, Lucia, Ramondetta, Alice, De Pasquale, Rocco, Sortino, Maria Angela, Merlo, Sara
Format:	Article
Language:	eng
Subjects:	Adenosine monophosphate-activated protein kinase Inflammation Nicotinamide phosphoribosyltransferase Psoriasis Silent information regulator 1
Online Access:	Get full text
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Summary:	Epidermal hyperplasia and the involvement of immune cells characterize the clinical picture of psoriasis. Among the several factors involved, attention has been focused on sirtuin 1 (SIRT1) - a deacetylase endowed with a variety of functions including the control of metabolic and inflammatory processes-, and on nicotinamide phosphoribosyltransferase (NAMPT), important for SIRT1 activation and involved in inflammatory events. The aim of the study was to analyze changes of SIRT1 and NAMPT expression in psoriatic skin. Samples from healthy controls and psoriatic patients were subjected to immunohistochemical analysis. A strong downregulation of SIRT1 expression was observed in skin samples from psoriatic patients compared to healthy controls. This was accompanied by a parallel reduction of adenosine monophosphate-activated kinase (AMPK) expression and, more strikingly, by the disappearance of cells immunolabeled for its active, phosphorylated form (pAMPK). In both cases, analysis of the distribution of immunopositive cells revealed a shift towards reduced intensity of staining. In contrast, NAMPT expression was upregulated in psoriatic samples in line with its pro-inflammatory role. This was again more visible with an intensity-based distribution analysis that evidenced a shift towards more intensely immunostained cell populations. The present data correlate in the same samples the expression of SIRT1, pAMPK/AMPK and NAMPT in psoriasis and open the way for novel pharmacological targets in the treatment of the disease.
ISSN:	1896-1126 1898-4002