Field Comparison of Removed Substrate Sampling and Emergence Traps for Estimating Culicoides Orbivirus Vectors in Northern Florida

The larval ecology of Culicoides (Diptera: Ceratopogonidae) influences their spatial distributions and the pathogens they transmit. These features are of special concern for deer farmers in Florida where epizootic hemorrhagic disease virus (EHDV) is a major source of mortality in captive herds. Rari...

Full description

Saved in:
Bibliographic Details
Published in:Journal of medical entomology 2022-09, Vol.59 (5), p.1660-1668
Main Authors: Black, Theodore Vincent, Quaglia, Agustin Ignacio, Wisely, Samantha, Burkett-Cadena, Nathan
Format: Article
Language:English
Subjects:
Analysis
Composition
Culicoides
Disease transmission
Ecological effects
EHDV
emergence trap
Health aspects
Hemorrhagic disease
Laboratories
larval ecology
Orbivirus
Plugs
Sampling
SAMPLING, DISTRIBUTION, DISPERSAL
Soils
Spatial distribution
Substrates
Traps
Vectors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The larval ecology of Culicoides (Diptera: Ceratopogonidae) influences their spatial distributions and the pathogens they transmit. These features are of special concern for deer farmers in Florida where epizootic hemorrhagic disease virus (EHDV) is a major source of mortality in captive herds. Rarity of larval morphological expertise leads many researchers to study larval ecology by quantifying emergence, either with field emergence traps or removing substrate from the field for observation under laboratory conditions. We investigated the comparability of these methods in Florida seepages where two recently implicated EHDV vectors, Culicoides stellifer Coquillett and Culicoides venustus Hoffman, are common. We compared the abundance and composition of emerging Culicoides collected from emergence traps with removed substrate samples (soil plugs) at three seepages. Soil plugs were sampled adjacent to the emergence trap and from underneath the trap footprint, and then monitored under laboratory conditions for 11–13 wk to compare the methods and to assess the role of incubation period for removed substrate samples. Emergence traps and removed substrate sampling largely agreed on community compositions and trends within different seepages. However, comparatively large numbers of C. stellifer emerged later than expected and well into the incubation period with emergence still occurring after 13 wk (90 d). Removed substrate samples were more similar to emergence traps at shorter incubation times. The importance of time for the capture of Culicoides in removed substrate sampling was more pronounced than we anticipated and is important from both a methodological and biological perspective.
ISSN:0022-2585
1938-2928
DOI:10.1093/jme/tjac089