Proteomic analysis of infected root canals with apical periodontitis in patients with type 2 diabetes mellitus: A cross‐sectional study

Aim This study aimed to quantitatively and qualitatively determine the proteomic profile of apical periodontitis (AP) in type 2 diabetes mellitus (T2DM) patients in comparison with systemically noncompromised patients and to correlate the protein expression of both groups with their biological funct...

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Bibliographic Details
Published in:International endodontic journal 2022-09, Vol.55 (9), p.910-922
Main Authors: Loureiro, Caroline, Buzalaf, Marília Afonso Rabelo, Pessan, Juliano Pelim, Ventura, Talita Mendes Oliveira, Pelá, Vinícius Taioqui, Ribeiro, Ana Paula Fernandes, Jacinto, Rogério de Castilho
Format: Article
Language:English
Subjects:
apical periodontitis
Apoptosis
Cross-sectional studies
Diabetes
diabetes mellitus
Diabetes mellitus (non-insulin dependent)
endodontics
Gelatinase
Gum disease
Histone H2B
host–pathogen interactions
Immune response
Immunoglobulins
Inflammation
Isoforms
Leukocytes (neutrophilic)
Lipocalin
Liquid chromatography
Mass spectroscopy
Neutrophils
Oxidative stress
Patients
Periodontitis
Prolactin
Protein expression
Proteins
Proteolysis
Proteomics
Root canals
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Summary:Aim This study aimed to quantitatively and qualitatively determine the proteomic profile of apical periodontitis (AP) in type 2 diabetes mellitus (T2DM) patients in comparison with systemically noncompromised patients and to correlate the protein expression of both groups with their biological functions. Methodology The sample consisted of 18 patients with asymptomatic AP divided into two groups according to the presence of T2DM: diabetic group—patients with T2DM (n = 9) and control group—systemically healthy patients (n = 9). After sample collection, the root canal samples were prepared for proteomic analysis using reverse‐phase liquid chromatography‐mass spectrometry. Label‐free quantitative proteomic analysis was performed by Protein Lynx Global Service software. Differences in protein expression between groups were calculated using t‐test (p 
ISSN:0143-2885
1365-2591
DOI:10.1111/iej.13794