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Identification of TCP family in moso bamboo (Phyllostachys edulis) and salt tolerance analysis of PheTCP9 in transgenic Arabidopsis
Main conclusion Bioinformatic analysis of moso bamboo TEOSINTE BRANCHED 1, CYCLOIDEA, and PROLIFERATING CELL FACTORS (TCP) transcription factors reveals their conservation and variation as well as the probable biological functions in abiotic stress response. Overexpressing PheTCP9 in Arabidopsis tha...
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Published in: | Planta 2022-07, Vol.256 (1), p.5-5, Article 5 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Main conclusion
Bioinformatic analysis of moso bamboo TEOSINTE BRANCHED 1, CYCLOIDEA, and PROLIFERATING CELL FACTORS (TCP) transcription factors reveals their conservation and variation as well as the probable biological functions in abiotic stress response. Overexpressing
PheTCP9
in
Arabidopsis thaliana
illustrates it may exhibit a new vision in different aspects of response to salt stress.
Plant specific TCPs play important roles in plant growth, development and stress response, but studies of TCP in moso bamboo are limited. Therefore, in this study, a total of 40 TCP genes (
PheTCP1
~
40
) were identified and characterized from moso bamboo genome and divided into three different subfamilies, namely, 7 in TEOSINTE BRANCHED 1 / CYCLOIDEA (TB1/CYC), 14 in CINCINNATA (CIN) and 19 in PROLIFERATING CELL FACTOR (PCF). Subsequently, we analyzed the gene structures and conserved domain of these genes and found that the members from the same subfamilies exhibited similar exon/intron distribution patterns. Selection pressure and gene duplication analysis results indicated that
PheTCP
genes underwent strong purification selection during evolution. There were many cis-elements related to phytohermone and stress responsive existing in the upstream promoter regions of
PheTCP
genes, such as ABRE, CGTCA-motif and ARE. Subcellular localization experiments showed that PheTCP9 was a nuclear localized protein. As shown by β-glucuronidase (GUS) activity, the promoter of
PheTCP9
was significantly indicated by salt stress.
PheTCP9
was significantly induced in the roots, stems and leaves of moso bamboo. It was also significantly induced by NaCl solution. Overexpressing
PheTCP9
increased the salt tolerance of transgenic
Arabidopsis
. Meanwhile, H
2
O
2
and malondialdehyde (MDA) contents were significantly lower in
PheTCP9
over expression (OE) transgenic
Arabidopsis
than WT. Catalase (CAT) activity, K
+
/Na
+
ratio as well as CAT2 expression level was also much improved in transgenic
Arabidopsis
than WT under salt conditions. In addition,
PheTCP9
OE transgenic
Arabidopsis
held higher survival rates of seedlings than WT under NaCl conditions. These results showed the positive regulation functions of
PheTCP9
in plants under salt conditions. |
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ISSN: | 0032-0935 1432-2048 |
DOI: | 10.1007/s00425-022-03917-z |