An insight into the morphology of DNA compaction induced by homobinuclear Ru(II) polypyridyl complexes

Binuclear Ru(II) polypyridyl complexes [Ru2(NN)4(BIPMB)]4+ (1–4), where N-N = 2,2′-bipyridine (bpy), 1,10-phenanthroline (phen), dipyrido [3,2-d:2′,3′-f] quinoxaline (dpq), and dipyrido[3,2-a:2′,3′-c]phenazine (dppz), have been synthesized using suitable precursors and bridging ligand (BIPMB), where...

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Published in:Journal of inorganic biochemistry 2022-09, Vol.234, p.111870-111870, Article 111870
Main Authors: Singh, Sushma B., Kumbhar, Avinash S., Walke, Gulshan, Kulkarni, Prasad P.
Format: Article
Language:English
Subjects:
Atomic force microscopy
Cytotoxicity
DNA condensation
Dynamic light scattering
Homobinuclear ruthenium (II) complex
Transmission electron microscopy
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Summary:Binuclear Ru(II) polypyridyl complexes [Ru2(NN)4(BIPMB)]4+ (1–4), where N-N = 2,2′-bipyridine (bpy), 1,10-phenanthroline (phen), dipyrido [3,2-d:2′,3′-f] quinoxaline (dpq), and dipyrido[3,2-a:2′,3′-c]phenazine (dppz), have been synthesized using suitable precursors and bridging ligand (BIPMB), where BIPMB = 3,3′-bis-{(imidazol-1-yl)-[4,5-f]-1,10-phenanthroline) methyl}-1,1′-biphenyl. The binding mode and affinity of complexes 1–4 with Calf Thymus DNA (CT-DNA) were determined by absorption and steady-state fluorescence spectroscopy. The decrease in viscosity of CT-DNA on sequential addition of these complexes indicated DNA condensation and the result was corroborated by circular dichroism (CD). The nanosized globular aggregates of CT-DNA induced by complexes 1–4 were observed by dynamic light scattering (DLS) and transmission electron microscopy (TEM) measurements. The gel electrophoretic mobility studies revealed the small orderly particles of supercoiled plasmid pBR322 DNA induced by these complexes. Additionally, the morphology and size of compact plasmid DNA condensates were studied by DLS and atomic force microscopy (AFM). The complexes were moderately cytotoxic against MCF-7 cells. A series of homobimetallic Ru(II) polypyridyl complexes efficiently induces DNA condensation. The morphology and size of small compact DNA condensates are thoroughly studied by nanoparticle characterization techniques such as Transmission Electron Microscopy, Atomic force microscopy and Dynamic light Scattering technique. [Display omitted] •A series of binuclear Ru(II) polypyridyl complexes were synthesized and characterized thoroughly.•The complexes efficiently induced DNA condensation on interaction with DNA.•The structural morphology of DNA condensates was studied by transmission electron microscopy.•The DNA condensates were also studied by atomic force microscopy and dynamic light scattering.•The complexes possessed moderate cytotoxicity against MCF-7 cells.
ISSN:0162-0134
1873-3344
DOI:10.1016/j.jinorgbio.2022.111870