Regulation of interleukin-6 and matrix metalloproteinases syntheses by bioflavonoids and photobiomodulation in human gingival fibroblasts

This study aimed to evaluate the separately effects of bioflavonoids proanthocyanidins, from grape seed extract (GSE) and synthetic naringenin (NA), as well as photobiomodulation (PBM) by low-level laser therapy on interleukin (IL)-6 and matrix metalloproteinases (MMPs) syntheses by human gingival f...

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Published in:Lasers in medical science 2022-09, Vol.37 (7), p.2973-2987
Main Authors: Cardoso, Laís Medeiros, Pansani, Taisa Nogueira, de Souza Costa, Carlos Alberto, Basso, Fernanda Gonçalves
Format: Article
Language:English
Subjects:
Bioflavonoids
Cell culture
Cell viability
Connective tissues
Cytokines
Cytotoxicity
Dentistry
Dimethyl sulfoxide
Exposure
Fibroblasts
Gelatinase A
Gelatinase B
Genotoxicity
Inflammation
Interleukin 6
Lasers
Light therapy
Matrix metalloproteinase
Matrix metalloproteinases
Medicine
Medicine & Public Health
Micronuclei
Naringenin
Optical Devices
Optics
Original Article
Photonics
Plant extracts
Quantum Optics
Seeds
Synthesis
Tumor necrosis factor-TNF
Tumor necrosis factor-α
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Summary:This study aimed to evaluate the separately effects of bioflavonoids proanthocyanidins, from grape seed extract (GSE) and synthetic naringenin (NA), as well as photobiomodulation (PBM) by low-level laser therapy on interleukin (IL)-6 and matrix metalloproteinases (MMPs) syntheses by human gingival fibroblasts (HGF). For this purpose, a connective tissue exposure (ulceration) model of HGF, stimulated with tumor necrosis factor-alpha (TNF-α), was used. Initially, the highest non-cytotoxic and non-genotoxic concentrations of bioflavonoids were determined by cell viability and micronuclei formation assays. Then, HGF were exposed to different stimuli: culture medium (negative control), dimethyl sulfoxide (DMSO), TNF-α, NA, GSE, TNF-α + NA, TNF-α + GSE, PBM (3 J/cm 2 , 0.025 W, 780 nm), and TNF-α + PBM. Next, IL-6, MMP-2, and MMP-9 syntheses were assessed. The concentration of 10 μg/mL of bioflavonoids increased cell viability at 24 and 48 h and did not present cytotoxic or genotoxic effects on HGF after 24, 48, and 72 h of contact. This concentration was selected for the assessment of bioflavonoids potential in modulating inflammatory mediators. TNF-α exposure enhanced IL-6 (170%), MMP-2 (10%), and MMP-9 (20%) syntheses, while a decrease of MMP-2 by 55% after exposure to TNF-α + GSE and 20% after TNF-α + NA and TNF-α + PBM was observed. MMP-9 synthesis was decreased by 35% after TNF-α + NA, 20% after TNF-α + GSE, and 30% after PBM. IL-6 was down-regulated by GSE in the presence of TNF-α (80%). In conclusion, TNF-α up-regulated IL-6 and MMPs, while bioflavonoids and PBM down-regulated MMP-2 and MMP-9 syntheses; GSE also decreased IL-6 synthesis, demonstrating the individual promising potential of these therapies for ulceration management.
ISSN:1435-604X
0268-8921
1435-604X
DOI:10.1007/s10103-022-03579-z