Moving beyond the state of the art of understanding resistance mechanisms in hookworms: confirming old and suggesting new associated SNPs

•Successful selection of an albendazole resistant hookworm strain.•Strong correlation between the SNP at codon 198 and drug resistance in hookworm.•Described for the first time, in hookworms, the presence of SNP A200L. Hookworms represent a serious problem for human and animal health in different pa...

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Published in:Acta tropica 2022-09, Vol.233, p.106533-106533, Article 106533
Main Authors: Medeiros, Celi da Silva, Furtado, Luis Fernando Viana, Miranda, Guilherme Silva, da Silva, Vivian Jordania, dos Santos, Talita Rodrigues, Rabelo, Élida Mara Leite
Format: Article
Language:English
Subjects:
Ancylostoma ceylanicum
Benzimidazoles
Beta-tubulin isotype-1 gene
Drug resistance
SNP
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Summary:•Successful selection of an albendazole resistant hookworm strain.•Strong correlation between the SNP at codon 198 and drug resistance in hookworm.•Described for the first time, in hookworms, the presence of SNP A200L. Hookworms represent a serious problem for human and animal health in different parts of the world. One of the suggested control strategies for parasitosis caused by members of the Ancylostomatidae family is mass drug aministration with benzimidazole compounds. This strategy has been proven to lead to the establishment of resistant strains in several nematodes related to SNPs at codons 167, 198 and 200 of the beta-tubulin isotype-1 gene. Through bioassay and in vivo analysis, we successfully isolated an albendazole-resistant A. ceylanicum strain by drug selective pressure. We observed a strong correlation between the presence of SNPs at codon 198 and drug resistance. We also described for the first time, in hookworms, the presence of SNP A200L, already described at low frequencies in ruminant nematodes. The results presented here are important for updating the current knowledge about anthelmintic resistance in hookworms. The answers and the new questions raised may provide a basis for the establishment of more effective control strategies. Graphical Abstract [Display omitted] .
ISSN:0001-706X
1873-6254
DOI:10.1016/j.actatropica.2022.106533