Human platelets release TGFBIp in acute myocardial infarction

Transforming growth factor-β-induced protein (TGFBIp) is released from activated platelets and promotes pro-thrombotic complications like pulmonary embolism. The role of TGFBIp in acute coronary syndrome, especially with a focus on platelets, has not been investigated so far. Using ELISA and immunob...

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Bibliographic Details
Published in:Heart and vessels 2022-11, Vol.37 (11), p.1962-1970
Main Authors: Kraemer, Bjoern F., Martinovic, Ivo, Lindemann, Stephan
Format: Article
Language:English
Subjects:
Acute coronary syndromes
Arteries
Biomedical Engineering and Bioengineering
Blood platelets
Cardiac Surgery
Cardiology
Collagen
Complications
Embolism
Enzyme-linked immunosorbent assay
Flow chambers
Growth factors
Heart attacks
Immunoblotting
Medicine
Medicine & Public Health
Myocardial infarction
Original Article
Platelets
Proteins
Vascular Surgery
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Summary:Transforming growth factor-β-induced protein (TGFBIp) is released from activated platelets and promotes pro-thrombotic complications like pulmonary embolism. The role of TGFBIp in acute coronary syndrome, especially with a focus on platelets, has not been investigated so far. Using ELISA and immunoblotting, we demonstrate platelet TGFBIp release in patients with myocardial infarction (MI). We investigated TGFBIp-induced platelet adhesion and rolling by flow chamber and chemotactic effects of TGFBIp in transwell experiments. Immunochemistry staining of arterial vessels detected TGFBIp and the platelet-specific protein GPVI in the vessel wall.We demonstrate for the first time that platelet TGFBIp release is significantly increased in MI and correlates with the severity of acute coronary syndromes (STEMI, NSTEMI). After activation with TRAP, platelets release TGFBIp and TGFBIp itself activates platelets. Under flow, TGFBIp-mediated platelet rolling and adherence similarly to collagen. TGFBIp significantly increased platelet transmigration and we demonstrate TGFBIp deposits in the wall of human arteries. In this study, we add novel aspects to the role of TGFBIp in acute coronary syndrome by demonstrating that TGFBIp is partially released from platelets during MI and has activating, pro-adhesive and pro-migratory effects on platelets that could contribute to the disease development of coronary vascular inflammation and MI.
ISSN:0910-8327
1615-2573
DOI:10.1007/s00380-022-02086-z