Regulation of Siglec-7-mediated varicella-zoster virus infection of primary monocytes by cis-ligands

Varicella-zoster virus (VZV) first infects hematopoietic cells, with the infected cells then acting to distribute the virus throughout the body. Sialic acid-binding immunoglobulin-like lectin (Siglec) family molecules recognize sialic acid-containing molecules on the same cell surface, called cis-li...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2022-07, Vol.613, p.41-46
Main Authors: Suenaga, Tadahiro, Mori, Yasuko, Suzutani, Tatsuo, Arase, Hisashi
Format: Article
Language:English
Subjects:
Cis-ligand
Entry receptor
Sialic acid
Siglec-7
Varicella-zoster virus
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Varicella-zoster virus (VZV) first infects hematopoietic cells, with the infected cells then acting to distribute the virus throughout the body. Sialic acid-binding immunoglobulin-like lectin (Siglec) family molecules recognize sialic acid-containing molecules on the same cell surface, called cis-ligands, or molecules on other cells or soluble agents, called trans-ligands. Among the Siglec family molecules, Siglec-4 and Siglec-7 mediate VZV infection through association with glycoprotein B (gB). As Siglec-7, but not Siglec-4, is expressed on hematopoietic cells such as monocytes, the regulatory mechanism by which Siglec-7 associates with gB is important to our understanding of VZV infection of blood cells. Here, we found that Siglec-7 is required for VZV to infect human primary monocytes. Furthermore, treatment of primary monocytes with sialidase enhanced both VZV gB binding to monocytes and VZV infectivity. Calcium influx in primary monocytes decreased the expression of Siglec-7 cis-ligands and increased VZV infectivity. These results demonstrate that the Siglec-7 cis-ligands present on primary monocytes play an important role in VZV infection through regulation of the interaction between gB and Siglec-7. •Siglec-7 is a major entry receptor for VZV to infect human primary monocytes.•Cis-ligands of Siglec-7 block the binding of Siglec-7 to VZV gB.•Cis-ligands of Siglec-7 protect VZV infection of human primary monocytes.•Siglec-7 cis-ligand expression on monocytes is regulated by intracellular Ca2+.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2022.04.111