Quantitative phase and refractive index imaging of 3D objects via optical transfer function reshaping

Deconvolution phase microscopy enables high-contrast visualization of transparent samples through reconstructions of their transmitted phases or refractive indexes. Herein, we propose a method to extend 2D deconvolution phase microscopy to thick 3D samples. The refractive index distribution of a sam...

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Bibliographic Details
Published in:Optics express 2022-04, Vol.30 (8), p.13802-13809
Main Authors: Hugonnet, Herve, Lee, Mahn Jae, Park, Yong Keun
Format: Article
Language:English
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Summary:Deconvolution phase microscopy enables high-contrast visualization of transparent samples through reconstructions of their transmitted phases or refractive indexes. Herein, we propose a method to extend 2D deconvolution phase microscopy to thick 3D samples. The refractive index distribution of a sample can be obtained at a specific axial plane by measuring only four intensity images obtained under optimized illumination patterns. Also, the optical phase delay of a sample can be measured using different illumination patterns.
ISSN:1094-4087
1094-4087
DOI:10.1364/oe.454533