Effects of cyclooxygenase and soluble epoxide hydrolase inhibitors on apoptosis of cultured primary equine chondrocytes

Apoptosis is an important mechanism underlying chondrocyte loss in osteoarthritis that could be affected by modulation of lipid signaling via inhibition of cyclooxygenases (COX) and soluble epoxide hydrolase (sEH). To determine the impact of inhibiting COX and sEH alone or in combination on apoptosi...

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Bibliographic Details
Published in:Research in veterinary science 2022-10, Vol.147, p.44-49
Main Authors: Walters, B., Trumble, T.N., Wendt-Hornickle, E., Kennedy, M., Guedes, AGP
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Biomedical materials
Cartilage
Caspase
Caspases
Chondrocyte
Chondrocytes
COX-inhibitors
Cyclooxygenase 2
Cytokines
Deprivation
Endoplasmic reticulum
Enzymes
Epoxide hydrolase
Epoxide Hydrolases
Horse
Horses
Hypothesis testing
In vivo methods and tests
Incubation
Inhibitors
Lipids
Metabolism
Metabolites
Osteoarthritis
Phenylbutazone
Software
Staphylococcal enterotoxin H
Stress
Tunicamycin
Variance analysis
Veterinary medicine
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Summary:Apoptosis is an important mechanism underlying chondrocyte loss in osteoarthritis that could be affected by modulation of lipid signaling via inhibition of cyclooxygenases (COX) and soluble epoxide hydrolase (sEH). To determine the impact of inhibiting COX and sEH alone or in combination on apoptosis of equine chondrocytes. Cultured primary equine chondrocytes were subjected to serum deprivation or incubation with 1 μg/ml tunicamycin for 24 h to induce apoptosis via caspase activation and endoplasmic reticulum (ER) stress, respectively. Cells were treated with the non-selective COX inhibitor phenylbutazone, the COX-2 selective inhibitor firocoxib and the sEH inhibitor t-TUCB alone or in combination. The inhibitors were used at half-maximal (IC50), 80% of maximal (IC80) and 10-fold the 80% inhibitory concentration (10xIC80) for the equine enzymes. Apoptosis was quantified via ELISA technique. Data were analyzed with unpaired two-tailed t-test or one-way ANOVA followed by Bonferroni's post-hoc while correcting for multiple comparisons via statistical hypothesis testing. P 
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2022.04.002