LINC00488 Induces Tumorigenicity in Retinoblastoma by Regulating microRNA-30a-5p/EPHB2 Axis

LINC00488 confers oncogenic activity in the progression of some tumors. Hence, the target of the study was about to specify LINC00488-mediated network in retinoblastoma (RB). LINC00488 expression was tested in RB clinical tissues. siRNA targeting LINC00488 or miR-30a-5p mimic was introduced into RB...

Full description

Saved in:
Bibliographic Details
Published in:Ocular immunology and inflammation 2023-03, Vol.31 (3), p.506-514
Main Authors: Cui, Xuehao, Liang, Tingyi, Ji, Xunda, Shao, Yan, Zhao, Peiquan, Li, Xiaorong
Format: Article
Language:English
Subjects:
Cell Line, Tumor
Cell Proliferation
Eph receptor B2
Humans
LINC00488
microRNA-30a-5p
MicroRNAs - metabolism
migration
proliferation
Receptor, EphB2 - metabolism
Retinal Neoplasms - metabolism
Retinal Neoplasms - pathology
retinoblastoma
Retinoblastoma - metabolism
Retinoblastoma - pathology
RNA, Long Noncoding - metabolism
tumorigenicity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:LINC00488 confers oncogenic activity in the progression of some tumors. Hence, the target of the study was about to specify LINC00488-mediated network in retinoblastoma (RB). LINC00488 expression was tested in RB clinical tissues. siRNA targeting LINC00488 or miR-30a-5p mimic was introduced into RB cell line (Y79) to observe cellular biological functions. The relationship between LINC00488, miR-30a-5p and EPHB2 was verified. Afterward, the role of miR-30a-5p involved in RB through targeted regulation of EPHB2 was probed in vitro and in vivo. LINC00488 was induced in RB tissue and cells. LINC00488 knockdown or miR-30a-5p upregulation depressed the malignant activities of Y79 cells. LINC00488 could sponge miR-30a-5p that targeted EPHB2. EPHB2, and EPHB2 overexpression counteracted miR-30a-5p restoration-induced inhibition of Y79 cell development in vitro and in vivo. LINC00488 induces tumorigenicity in RB by binding to miR-30a-5p to target EPHB2, which may offer a new clue of RB treatment from an lncRNA-miRNA-mRNA network.
ISSN:0927-3948
1744-5078
DOI:10.1080/09273948.2022.2037659