Autophagy induced by largemouth bass virus inhibits virus replication and apoptosis in epithelioma papulosum cyprini cells

Autophagy and apoptosis play important roles in the occurrence and development of diseases. Largemouth bass virus (LMBV) is a primary agent that causes infectious skin ulcerative syndrome in largemouth bass and threatens the aquaculture of the species. We investigated the relationship between LMBV a...

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Bibliographic Details
Published in:Fish & shellfish immunology 2022-04, Vol.123, p.489-495
Main Authors: Deng, Lishuang, Feng, Yang, OuYang, Ping, Chen, Defang, Huang, Xiaoli, Guo, Hongrui, Deng, Huidan, Fang, Jing, Lai, Weimin, Geng, Yi
Format: Article
Language:English
Subjects:
Animals
Antivirus
Apoptosis
Autophagy
Bass
Carcinoma
Cyprinidae
DNA Virus Infections
DNA Viruses
Fish Diseases
Largemouth bass virus
Replication
Virus Replication
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Summary:Autophagy and apoptosis play important roles in the occurrence and development of diseases. Largemouth bass virus (LMBV) is a primary agent that causes infectious skin ulcerative syndrome in largemouth bass and threatens the aquaculture of the species. We investigated the relationship between LMBV and autophagy, as well as the effect of autophagy on apoptosis induced by LMBV. Results showed that LMBV could induce autophagy in epithelioma papulosum cyprinid (EPC) cells. There was also an increase in LC3-II protein and decrease in p62 protein, along with autophagosome-like membranous vesicles and punctate autophagosomes fluorescent spots being observed in EPC cells. Enhancing autophagy inhibited the replication of LMBV and apoptosis in EPC cells while inhibiting autophagy produced the opposite effect. These results offer new insights into the pathogenesis of LMBV and anti-LMBV strategies. [Display omitted] •LMBV induced autophagy in EPC cells.•Autophagy inhibited LMBV replication and extracellular viral yields.•Autophagy inhibited apoptosis induced by LMBV in EPC cells.
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2022.03.026