Antihyperglycemic effect of an anthocyanin, cyanidin-3- O -glucoside, is achieved by regulating GLUT-1 via the Wnt/β-catenin-WISP1 signaling pathway

Cyanidin-3- -glucoside (C3G), an essential representative of anthocyanins, has been proved to possess a myriad of biological activities. However, the effects of C3G on glucose metabolism and its underlying molecular mechanisms remain elusive. The aim of the present study was to investigate the metab...

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Bibliographic Details
Published in:Food & function 2022-04, Vol.13 (8), p.4612-4623
Main Authors: Ye, Xiang, Chen, Wen, Tu, Pengcheng, Jia, Ruoyi, Liu, Yangyang, Tang, Qiong, Chen, Chuan, Yang, Caihong, Zheng, Xiaodong, Chu, Qiang
Format: Article
Language:English
Subjects:
Animals
Anthocyanins
Anthocyanins - pharmacology
beta Catenin - genetics
Diabetes
Diabetes mellitus
Glucose
Glucose metabolism
Glucose transporter
Glucosides
Glucosides - pharmacology
Glycogen
Glycogens
Hepatocytes
Hypoglycemic Agents - pharmacology
In vivo methods and tests
Liver
Mice
Molecular docking
Molecular Docking Simulation
Molecular modelling
Signal Transduction
Signaling
Wnt protein
β-Catenin
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Summary:Cyanidin-3- -glucoside (C3G), an essential representative of anthocyanins, has been proved to possess a myriad of biological activities. However, the effects of C3G on glucose metabolism and its underlying molecular mechanisms remain elusive. The aim of the present study was to investigate the metabolic impact of C3G on db/db mice and to determine whether its consequent anti-diabetic effects were related to glucose transporter-1 (GLUT-1) by and studies. As a result, through diabetic db/db mice, C3G treatment was found to significantly reduce the fasting blood glucose level and increase glycogen synthesis, which were associated with upregulation of GLUT-1 expression in the liver of the mice. In addition, in liver cells of the HepG2 and L02 lines, we further discovered that C3G could effectively promote glucose consumption by regulating the Wnt/β-catenin-WISP1 signaling pathway. Nevertheless, such effects would be restricted when the expression of GLUT-1 was blocked by the inhibitor IWR-1. Meanwhile, molecular docking technology was applied to simulate the possible action sites of C3G at the molecular level, and the results indicated that C3G might bind to β-catenin. In conclusion, our study provided evidence of the antihyperglycemic effect of C3G and regulating GLUT-1 expression and the related signaling pathways.
ISSN:2042-6496
2042-650X
DOI:10.1039/d1fo03730g