Simultaneous determination of dimethoate and its metabolite omethoate in curry leaf using LC‐MS/MS and risk assessment

This study presents the method development, validation, and simultaneous determination of dimethoate and its metabolite omethoate in curry leaf. Samples were extracted following modified quick, easy, cheap, effective, rugged, and safe extraction protocol and analyzed using liquid chromatography‐tand...

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Bibliographic Details
Published in:Journal of separation science 2022-06, Vol.45 (11), p.1831-1838
Main Authors: Pasar, Ratnamma, MS, Pallavi, R, Harischandra Naik, M, Devaraj, P, Nandini, M, Bheemanna, R, Badariprasad P, M, Paramasivam
Format: Article
Language:English
Subjects:
Acetonitrile
curry leaf
dimethoate
dissipation
Liquid chromatography
Mass spectrometry
Metabolites
omethoate
Reproducibility
Risk assessment
Standard deviation
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Summary:This study presents the method development, validation, and simultaneous determination of dimethoate and its metabolite omethoate in curry leaf. Samples were extracted following modified quick, easy, cheap, effective, rugged, and safe extraction protocol and analyzed using liquid chromatography‐tandem mass spectrometry. The limit of quantification in the matrix was 0.005 μg g−1 for dimethoate and omethoate. Extraction using acetonitrile recorded the average recoveries in the range of 82.25 to 112.97% for dimethoate and 85.57 to 107.22% for omethoate at 0.005, 0.025 and 0.050 μg g−1 fortification levels and relative standard deviation less than 5%. Similarly, the relative standard deviation values for intraday (Repeatability) and interday (Reproducibility) tests were less than 15%. Dissipation kinetics of dimethoate 30% emulsifiable concentrate at 200 and 400 g a.i h−1 recorded initial deposits of 5.20 and 10.05 μg g−1 and 0.33 and 0.48 μg g−1 for dimethoate and omethoate, respectively, and half‐life of 3.07 and 3.34 days. The estimated hazard index value found more than one at a day after dimethoate application. It is not safe for consumer health to use curry leaves in the initial days after application.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.202100696