A graphene-based electrochemical flow analysis device for simultaneous determination of dopamine, 5-hydroxytryptamine, and melatonin

Developing an accurate, sensitive, and efficient portable device for simultaneous detection of disease-related biomolecules is an attractive challenge for point-of-care testing (POCT). In this work, we introduced a novel graphene-based electrochemical flow analysis device (GEFAD), which combined a 3...

Full description

Saved in:
Bibliographic Details
Published in:Analyst (London) 2022-04, Vol.147 (8), p.1598-161
Main Authors: Deng, Huizhen, Zhao, Jie, Zhao, Shifan, Jiang, Shuai, Cui, Guofeng
Format: Article
Language:English
Subjects:
Ascorbic Acid - chemistry
Biomolecules
Dopamine
Dopamine - analysis
Electrochemical analysis
Electrochemical Techniques - methods
Electrodes
Gold
Gold - chemistry
Graphene
Graphite - chemistry
Melatonin
Metal Nanoparticles - chemistry
Nanoparticles
Portable equipment
Reproducibility
Reproducibility of Results
Selectivity
Serotonin
Three dimensional flow
Three dimensional printing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Developing an accurate, sensitive, and efficient portable device for simultaneous detection of disease-related biomolecules is an attractive challenge for point-of-care testing (POCT). In this work, we introduced a novel graphene-based electrochemical flow analysis device (GEFAD), which combined a 3D-printed flow cell with a three-electrode system based on a gold nanoparticle-modified graphene (AuNPs@Gr) working electrode to simultaneously detect depression-related biomolecules: dopamine (DA), 5-hydroxytrypserotonin (5-HT), and melatonin (MEL). Electrochemical studies showed that the GEFAD had high selectivity and sensitivity in the determination of DA, 5-HT, and MEL with their significant separation peaks. The linear ranges of DA, 5-HT, and MEL were respectively found to be 0.1-70 μM, 0.1-100 μM, and 0.1-150 μM, with the corresponding detection limits of 0.054 μM, 0.071 μM, and 0.087 μM (S/N = 3). In addition, the GEFAD presented a good anti-interference ability and satisfactory reproducibility, and can be used in real calf serum sample analysis. The introduced in-flow electrochemical detection device showed excellent repeatability, less sample consumption, and simple operation, which increases the possibility to implement POCT. This study also proposed a promising application prospect for the development of portable biomolecules detection devices. A graphene-based electrochemical flow analysis device for simultaneous determination of dopamine, 5-hydroxytryptamine, and melatonin.
ISSN:0003-2654
1364-5528
DOI:10.1039/d1an02318g