Redox mediated biosensors incorporating cultured fish cells for toxicity assessment

Fish are an important genus within ecosystems, and practical sensing devices, incorporating fish cells, could be used with advantage for environmental monitoring and protection. In this paper, redox mediated biosensors were prepared by immobilizing cultured fish cells at a carbon electrode surface....

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Bibliographic Details
Published in:Biosensors & bioelectronics 1996, Vol.11 (12), p.1253-1257
Main Authors: Polak, Monika E., Rawson, David M., Haggett, Barry G.D.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensors
Biotechnology
Cell culture
Cell immobilization
Cells
Chemicals
cultured fish cells
Ecosystems
Fisheries
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
redox mediator
Risk assessment
Toxicity
toxicity assessment
Various methods and equipments
whole cell biosensor
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Summary:Fish are an important genus within ecosystems, and practical sensing devices, incorporating fish cells, could be used with advantage for environmental monitoring and protection. In this paper, redox mediated biosensors were prepared by immobilizing cultured fish cells at a carbon electrode surface. EPC (from carp) and BF-2 (from bluegill sunfish) cells could be monitored by using a lipophilic mediator (e.g. 2,6-dimethylbenzoquinone) added to the solution bathing the sensor. Currents measured in the external circuit were indicative of the metabolic activity of the immobilized cells and the sensors could be used to determine the presence of chemicals within the bathing medium that perturbed their normal metabolic status.
ISSN:0956-5663
1873-4235
DOI:10.1016/0956-5663(96)88090-0