Lactoferrin-loaded nanostructured lipid carriers (NLCs) as a new formulation for optimized ocular drug delivery

[Display omitted] Nanostructured lipid carriers (NLC) are novel lipidic nanosystems that provide significant improvements in terms of high drug loading capacity and controlled drug release. The purpose of the present work was based on the design, development, and physicochemical characterization of...

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Published in:European journal of pharmaceutics and biopharmaceutics 2022-03, Vol.172, p.144-156
Main Authors: Varela-Fernández, Rubén, García-Otero, Xurxo, Díaz-Tomé, Victoria, Regueiro, Uxía, López-López, Maite, González-Barcia, Miguel, Isabel Lema, María, Javier Otero-Espinar, Francisco
Format: Article
Language:English
Subjects:
Double emulsification-solvent evaporation method
Keratoconus
Lactoferrin
Nanostructured lipid carriers (NLC)
Ocular drug delivery
Topical ophthalmic administration
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Summary:[Display omitted] Nanostructured lipid carriers (NLC) are novel lipidic nanosystems that provide significant improvements in terms of high drug loading capacity and controlled drug release. The purpose of the present work was based on the design, development, and physicochemical characterization of lactoferrin-loaded NLCs as a new therapeutic alternative for the keratoconus treatment. Lactoferrin-loaded NLCs were successfully prepared by a double emulsion/solvent evaporation method. The resultant NLC were assessed in terms of particle size, size distribution, surface charge, morphology, encapsulation efficiency (EE), loading capacity (LC), stability, cytotoxicity, in vitro release, and ocular surface retention. Resulting data showed a size of 119.45 ± 11.44 nm, a 0.151 ± 0.045 PDI value and a surface charge of −17.50 ± 2.53 mV. Besides, high EE and LC values were obtained (up to 75%). The in vitro release study demonstrated a lactoferrin controlled release pattern. NLCs were also stable, non-toxic and show mucoadhesive properties. Thus, a consistent preclinical base was obtained, where NLC may be considered as a potential controlled release novel drug delivery system of lactoferrin for the keratoconus treatment.
ISSN:0939-6411
1873-3441
DOI:10.1016/j.ejpb.2022.02.010