Molecular evolution and functional characterization of chitinase gene family in Populus trichocarpa

•Forty-eight Populus chitinases were identified and classified into seven groups.•The Populus chitinase gene family expanded primarily through tandem duplication.•The Populus and Arabidopsis chitinase families have undergone different expansion.•The expression of the Populus chitinase gene family is...

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Bibliographic Details
Published in:Gene 2022-05, Vol.822, p.146329-146329, Article 146329
Main Authors: Zhang, Yuan-Jie, Ren, Lin-Ling, Lin, Xiao-Yang, Han, Xue-Min, Wang, Wei, Yang, Zhi-Ling
Format: Article
Language:English
Subjects:
Chitinase
Chitinases - genetics
Chitinases - metabolism
Chromosome Mapping - methods
Evolution, Molecular
Functional divergence
Gene Expression Regulation, Plant
Multigene Family
Phylogeny
Plant Leaves - enzymology
Plant Leaves - genetics
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Roots - enzymology
Plant Roots - genetics
Plant Stems - enzymology
Plant Stems - genetics
Poplar
Populus - enzymology
Populus - genetics
Selection, Genetic
Stress response
Tandem duplication
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Summary:•Forty-eight Populus chitinases were identified and classified into seven groups.•The Populus chitinase gene family expanded primarily through tandem duplication.•The Populus and Arabidopsis chitinase families have undergone different expansion.•The expression of the Populus chitinase gene family is divergent and inducible. Chitinases, the chitin-degrading enzymes, have been shown to play important role in defense against the chitin-containing fungal pathogens. In this study, we identified 48 chitinase-coding genes from the woody model plant Populus trichocarpa. Based on phylogenetic analysis, the Populus chitinases were classified into seven groups. Different gene structures and protein domain architectures were found among the seven Populus chitinase groups. Selection pressure analysis indicated that all the seven groups are under purifying selection. Phylogenetic analysis combined with chromosome location analysis showed that Populus chitinase gene family mainly expanded through tandem duplication. The Populus chitinase gene family underwent marked expression divergence and is inducibly expressed in response to treatments, such as chitosan, chitin, salicylic acid and methyl jasmonate. Protein enzymatic activity analysis showed that Populus chitinases had activity towards both chitin and chitosan. By integrating sequence characteristic, phylogenetic, selection pressure, gene expression and protein activity analysis, this study shed light on the evolution and function of chitinase family in poplar.
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2022.146329