Protein Patterning on Microtextured Polymeric Nanobrush Templates Obtained by Nanosecond Fiber Laser

Micropatterned polymer brushes have attracted attention in several biomedical areas, i.e., tissue engineering, protein microarray, biosensors, etc., for precise arrangement of biomolecules. Herein, a facile and scalable approach is reported to create microtextured polymer brushes with the ability to...

Full description

Saved in:
Bibliographic Details
Published in:Macromolecular bioscience 2022-05, Vol.22 (5), p.e2100454-n/a
Main Authors: Verma, Meenakshi, Rana, Abhishek, Vidyasagar, Koyyana E. Ch, Kalyanasundaram, Dinesh, Saha, Sampa
Format: Article
Language:English
Subjects:
Ablation
Alloys
Atomic force microscopy
Biomedical engineering
Biomolecules
Biosensors
Brushes
Conjugation
Fiber lasers
Fluorescence
Fluorescence microscopy
Initiators
laser ablation
Lasers
Medical research
Micropatterning
Microscopy
Microscopy, Atomic Force
Photoelectron spectroscopy
Photoelectrons
Polyethylene glycol
polymer brush
Polymerization
Polymers
Polymers - chemistry
Protein adsorption
Protein arrays
Protein engineering
Proteins
Substrates
surface initiated atom transfer radical polymerization
Surface Properties
Tissue engineering
titanium
Titanium base alloys
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Micropatterned polymer brushes have attracted attention in several biomedical areas, i.e., tissue engineering, protein microarray, biosensors, etc., for precise arrangement of biomolecules. Herein, a facile and scalable approach is reported to create microtextured polymer brushes with the ability to generate different type of protein patterns. Nanosecond fiber laser is exploited to generate micropatterns on poly(poly(ethylene glycol) methacrylate) (polyPEGMA) brush modified Ti alloy substrate. Surface initiated atom transfer radical polymerization is employed to grow PolyPEGMA brush (11–87 nm thick) on Ti alloy surface immobilized with initiator having an initiator density (σ*) of 1.5 initiators per nm2. Polymer brushes are then selectively laser ablated and their presence on nontextured area is confirmed by atomic force microscopy, fluorescence microscopy, and X‐ray photoelectron spectroscopy. Spatial orientation of biomolecules is first achieved by nonspecific protein adsorption on areas ablated by the laser, via physisorption. Further, patterned brushes of polyPEGMA are modified to activated ester that gives rise to protein conjugation specifically on nonlaser ablated brush areas. Moreover, the laser ablated brush modified patterned template is also successfully utilized for generating alternate patterns of bacteria. This promising technique can be further extended to create interesting patterns of several biomolecules which are of great interest to biomedical research community. A novel technique utilizing nanosecond pulse laser on poly(ethylene glycol) methacrylate (polyPEGMA) brush modified Titanium alloy (Ti6Al4V, Tia) is employed to generate patterns on Titanium surface for demonstrating protein patterning through specific and nonspecific adsorption approach. Additionally, the textured surfaces are exploited for generating alternate patterns of bacteria. Bacteria predominantly recognizes the surface chemical signal to stay majorly on line patterns devoid of polymer brushes.
ISSN:1616-5187
1616-5195
DOI:10.1002/mabi.202100454