A bacterial chemoreceptor that mediates chemotaxis to two different plant hormones

Summary Indole‐3‐acetic acid (IAA) is the main naturally occurring auxin and is produced by organisms of all kingdoms of life. In addition to the regulation of plant growth and development, IAA plays an important role in the interaction between plants and growth‐promoting and phytopathogenic bacteri...

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Bibliographic Details
Published in:Environmental microbiology 2022-08, Vol.24 (8), p.3580-3597
Main Authors: Rico‐Jiménez, Miriam, Roca, Amalia, Krell, Tino, Matilla, Miguel A.
Format: Article
Language:English
Subjects:
Acetic acid
Aromatic compounds
Auxins
Bacteria
Chemoreceptors
Chemotaxis
Gene expression
Hormones
Indoleacetic acid
Metabolism
Pathogens
Phytohormones
Plant bacterial diseases
Plant growth
Plant growth substances
Plants
Pseudomonas putida
Receptors
Salicylic acid
Taxis
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Summary:Summary Indole‐3‐acetic acid (IAA) is the main naturally occurring auxin and is produced by organisms of all kingdoms of life. In addition to the regulation of plant growth and development, IAA plays an important role in the interaction between plants and growth‐promoting and phytopathogenic bacteria by regulating bacterial gene expression and physiology. We show here that an IAA metabolizing plant‐associated Pseudomonas putida isolate exhibits chemotaxis to IAA that is independent of auxin metabolism. We found that IAA chemotaxis is based on the activity of the PcpI chemoreceptor and heterologous expression of pcpI conferred IAA taxis to different environmental and human pathogenic isolates of the Pseudomonas genus. Using ligand screening, microcalorimetry and quantitative chemotaxis assays, we found that PcpI failed to bind IAA directly, but recognized and mediated chemoattractions to various aromatic compounds, including the phytohormone salicylic acid. The expression of pcpI and its role in the interactions with plants was also investigated. PcpI extends the range of central signal molecules recognized by chemoreceptors. To our knowledge, this is the first report on a bacterial receptor that responds to two different phytohormones. Our study reinforces the multifunctional role of IAA and salicylic acid as intra‐ and inter‐kingdom signal molecules.
ISSN:1462-2912
1462-2920
1462-2920
DOI:10.1111/1462-2920.15920