Green efficient ultrasonic-assisted extraction of abalone nacre water-soluble organic matrix for bioinspired enamel remineralization

Reconstructing enamel-like hydroxyapatite structures on damaged teeth remains a great challenge in the materials science and dentistry due to its highly ordered hierarchical microstructure. Inspired by the mineralization of mollusk nacre in nature, abalone nacre water-soluble organic matrix (WSM) wa...

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Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2022-04, Vol.212, p.112336-112336, Article 112336
Main Authors: Xing, Huaran, Yang, Faming, Sun, Shengjun, Pan, Panpan, Wang, Hongbo, Wang, Yuxin, Chen, Jingdi
Format: Article
Language:English
Subjects:
Abalone nacre, Water-soluble organic matrix
Durapatite - chemistry
Durapatite - pharmacology
Enamel remineralization
Enamel-like hydroxyapatite
Nacre - chemistry
Tooth Remineralization - methods
Ultrasonics
Ultrasound-assisted water extraction
Water
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Summary:Reconstructing enamel-like hydroxyapatite structures on damaged teeth remains a great challenge in the materials science and dentistry due to its highly ordered hierarchical microstructure. Inspired by the mineralization of mollusk nacre in nature, abalone nacre water-soluble organic matrix (WSM) was isolated successfully though an ultrasonic-assisted water extraction (UWE) strategy with nondestructive activity and high-quality extraction for simulating the process of tooth hard tissue mineralization. Results showed that the UWE strategy significantly increased the protein yield from 7.60% to 9.60% and improved the polysaccharide yield from 2.59% to 3.34%, respectively, indicating its excellent extraction efficiency of WSM. Noteworthily, the smallest averaged particle size (~155 nm) of WSM were obtained at an ultrasound time of 6 h, whereas the highest absolute values (~ −32 mV) of zeta potential was produced. Moreover, it was proved that WSM could induce the growth of enamel-like hydroxyapatite crystals to further facilitate biomimetic remineralization of the demineralized enamel and restore its continuous and smooth surface structure in vitro. Besides, the hardness (4.37 ± 0.07 GPa) and modulus of elasticity (84.80 ± 1.49 GPa) of the WSM-repaired enamel was similar to that of native enamel, indicating superior mechanical properties after repair. Herein, it provides a promising green, efficient strategy for the remineralization of damaged enamel and high value utilization of waste abalone shells. [Display omitted] •Large numbers of abandoned abalone shells resulted in resource waste and even environmental pollution.•Similarity of calcium salt deposition between abalone shell and tooth.•Abalone nacre water-soluble organic matrix (WSM) as an organic template could simulate the synthesis of enamel-like HA.•A green and efficient ultrasonic-assisted water extraction (UWE) strategy was developed to isolate successfully WSM.•WSM facilitated biomimetic remineralization of the demineralized enamel.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2022.112336