Synthesis, physico-chemical properties and effect of adenosine thiamine triphosphate on vitamin B1 metabolism in the liver of alloxan diabetic rats

Adenosine thiamine triphosphate (AThTP) is a nucleotide discovered in bacteria and some other living organisms more than a decade ago. No biochemical function for AThTP has been established yet, however, experimental data available indicate its possible involvement in metabolic regulation or cell si...

Full description

Saved in:
Bibliographic Details
Published in:Biochimica et biophysica acta. General subjects 2022-04, Vol.1866 (4), p.130086-130086, Article 130086
Main Authors: Makarchikov, Alexander F., Kudyrka, Tatsiana G., Luchko, Tatyana A., Yantsevich, Aliaksei V., Rusina, Iryna M., Makar, Alena A., Kolas, Iryna K., Usanov, Sergey A.
Format: Article
Language:English
Subjects:
Adenosine thiamine triphosphate
Alloxan-induced diabetes
Rat liver
Thiamine
Vitamin B1
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Adenosine thiamine triphosphate (AThTP) is a nucleotide discovered in bacteria and some other living organisms more than a decade ago. No biochemical function for AThTP has been established yet, however, experimental data available indicate its possible involvement in metabolic regulation or cell signaling. Metabolism of AThTP in mammals, as well as the feasibility of its pharmacological application, is essentially unstudied. Preparative low-pressure chromatography was employed to purify chemically synthesized AThTP with its further analysis by mass spectrometry, HPLC, UV and fluorescence spectroscopy. Enzyme activity assays along with HPLC were used to examine the effects of AThTP and thiamine on vitamin B1 metabolism in the liver of alloxan-induced diabetic rats. An improved procedure for AThTP synthesis and purification is elaborated. Solution stability, optical spectral properties and the molar absorption coefficient for AThTP were determined. The levels of thiamine compounds were found to be increased in the liver of diabetic rats. Neither AThTP nor thiamine treatment affected hepatic vitamin B1 metabolism. Fasting blood glucose concentration was also unchangeable after AThTP or thiamine administration. Contrast to the widespread view about thiamine deficiency in diabetes, our results clearly shows an adaptive increase in the level of B1 vitamers in the liver of alloxan diabetic rats with no further rising after AThTP or thiamine treatment at a moderate dose. Neither AThTP nor thiamine is effective in glycaemic control. These findings are to be considered in future studies dealing with thiamine or its analogues application to correct metabolic disturbances in diabetes. •A method for adenosine thiamine triphosphate (AThTP) preparation is elaborated.•Spectral properties and the molar absorption coefficient for AThTP are determined.•No thiamine deficiency occurs in the liver of alloxan-induced diabetic rats.•Diabetes causes increasing the levels of B1 vitamers in rat liver.•AThTP or thiamine treatment did not affect hepatic B1 metabolism in diabetic rats.
ISSN:0304-4165
1872-8006
DOI:10.1016/j.bbagen.2022.130086