Prognostic significance of CRLF2 overexpression and JAK2 mutation in Egyptian pediatric patients with B-precursor acute lymphoblastic leukemia

The prognostic significance of cytokine receptor-like factor 2 (CRLF2) overexpression in pediatric B-cell precursor (BCP) acute lymphoblastic leukemia (ALL) is still controversial. We aimed to investigate the role of CRLF2 overexpression and JAK2 mutation in the diagnosis and prognosis of newly diag...

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Published in:Clinical lymphoma, myeloma and leukemia myeloma and leukemia, 2022-06, Vol.22 (6), p.e376-e385
Main Authors: Hassan, Naglaa M., Abdellateif, Mona S., Radwan, Enas M., Hameed, Sayed Abed, Desouky, Eman D. El, Kamel, Mahmoud M., Gameel, Abdallah M.
Format: Article
Language:English
Subjects:
BCP-ALL
Burkitt Lymphoma
Child
CRLF2
Egypt
Humans
JAK2 R683G
Janus Kinase 2 - genetics
Mutation
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - diagnosis
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - genetics
Precursor Cell Lymphoblastic Leukemia-Lymphoma - diagnosis
Precursor Cell Lymphoblastic Leukemia-Lymphoma - genetics
Prognosis
Receptors, Cytokine - genetics
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Summary:The prognostic significance of cytokine receptor-like factor 2 (CRLF2) overexpression in pediatric B-cell precursor (BCP) acute lymphoblastic leukemia (ALL) is still controversial. We aimed to investigate the role of CRLF2 overexpression and JAK2 mutation in the diagnosis and prognosis of newly diagnosed pediatric B-ALL patients. CRLF2 expression was assessed by real-time quantitative polymerase chain reaction (PCR) in 115 pediatric patients newly diagnosed with precursor B-ALL patients compared with 24 age- and sex-matched controls. JAK2 R683G mutation status was performed by the qBiomarker Somatic Mutation PCR Assay. CRLF2 overexpression was identified in 21 patients (18.3%), while the JAK2 R683G mutant type was found in only in 7 patients (6.1%). There was a significant CRLF2 overexpression in patients with high initial TLC, high blast count in blood, and organomegaly (P .04, 0.005 & 0.05 respectively). No patients with CRLF2 overexpression expressed any recurrent cytogenetic translocations. 4 patients with CRLF2 overexpression showed JAK2 R683G mutation. CRLF2 levels and JAK2 R683G mutation status did not have a significant impact on either overall survival or disease-free survival. CRLF2 expression was significantly higher in Egyptian precursor B-ALL pediatric patients. CRLF2 overexpression was associated with a number of unfavorable prognostic factors with high tumor load, but was not an adverse independent parameter in pediatric BCP-ALL patients. Some patients with CRLF2 overexpression display JAK2 mutation, which may benefit from targeted therapy by kinase inhibitors. Role of CRLF2 overexpression and JAK2 mutation in newly diagnosed Egyptian pediatric B-ALL patients. 115 patients and 24 controls were assessed by RT-qPCR assays for CRLF2 and JAK2 R683G mutation status. CRLF2 overexpression was not an independent adverse prognostic parameter in our pediatric B-ALL. Patients with CRLF2 overexpression may harbor JAK2 mutations, presenting a subset that can benefit from specific therapeutic strategies targeting these abnormalities, which may improve the prognosis of B-ALL.
ISSN:2152-2650
2152-2669
DOI:10.1016/j.clml.2021.12.006