Folate functionalized chitosan nanoparticles as targeted delivery systems for improved anticancer efficiency of cytarabine in MCF-7 human breast cancer cell lines

•Cytarabine loaded folate conjugated chitosan nanoparticles were prepared by ionic gelation method.•Positive zeta potential of synthesised FCCNPs indicates a stable nanoformulation.•Cell uptake of FCCNPs was tracked with the aid of fluorophore, Squaraine 650.•Treatment with FCCNPs upregulates genes,...

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Bibliographic Details
Published in:International journal of biological macromolecules 2022-02, Vol.199, p.150-161
Main Authors: Geethakumari, Deepa, Bhaskaran Sathyabhama, Anoop, Raji Sathyan, Krishnapriya, Mohandas, Dhaneesha, Somasekharan, Jisha V., Thavarool Puthiyedathu, Sajeevan
Format: Article
Language:English
Subjects:
A-549 cells
Breast Neoplasms - pathology
Cell Survival
Chitosan
Chitosan - therapeutic use
Cytarabine
Cytarabine - pharmacology
Drug Carriers - therapeutic use
Drug Delivery Systems
Female
Folate receptors
Folic Acid
Humans
MCF-7 Cells
Nanoparticles
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Summary:•Cytarabine loaded folate conjugated chitosan nanoparticles were prepared by ionic gelation method.•Positive zeta potential of synthesised FCCNPs indicates a stable nanoformulation.•Cell uptake of FCCNPs was tracked with the aid of fluorophore, Squaraine 650.•Treatment with FCCNPs upregulates genes, bax, cyt c and cas 9 involved in apoptosis.•Folate conjugated chitosan nanoparticles showed improved cytotoxicity of anticancer drug, cytarabine in MCF-7 cell lines. Anticancer drug cytarabine, has been widely used for treating haematological malignancies while it has minimal activity against solid tumours, which demands continuous infusion leading to high dose cytarabine toxicity. In this study, folate conjugated chitosan nanoparticles (FCCNP) were used for targeted delivery of cytarabine in breast adenocarcinoma cell lines by making use of the overexpressed folate receptors on the surface of MCF-7. Folate was conjugated to chitosan using carbodiimide. FCCNPs show spherical morphology with a size of
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2021.12.070