Plasminogen gene polymorphisms [c.924C>T and IVS 8+14 G>A] in periodontitis and familial Mediterranean fever: A case‐control study

Background and objective The plasminogen (PLG) activation system plays an essential role in severe inflammation based diseases such as periodontitis, destructive membranous periodontal disease (ligneous periodontitis), familial Mediterranean fever (FMF), and amyloidosis. We have aimed to evaluate va...

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Published in:Journal of periodontal research 2022-04, Vol.57 (2), p.371-380
Main Authors: Fentoğlu, Özlem, Tülüceoğlu, Eda Evgen, Orhan, Hikmet
Format: Article
Language:English
Subjects:
Amyloid
Amyloidosis
Case-Control Studies
Cross-Sectional Studies
DNA sequencing
Enzyme-linked immunosorbent assay
Exons
Familial Mediterranean fever
Familial Mediterranean Fever - genetics
Gene frequency
Gene Frequency - genetics
Gene polymorphism
genetics
Genotype
Genotype & phenotype
Gum disease
Health care
Heterozygotes
Humans
inflammasome
Inflammatory diseases
Morbidity
Mutation
Patients
Periodontal diseases
Periodontitis
Periodontitis - complications
Periodontitis - genetics
Plasminogen - genetics
plasminogen gene
PLG, MEFV
Polymorphism
Polymorphism, Genetic - genetics
Public health
Pyrin - genetics
Pyrin protein
Quality of Life
Serum levels
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Summary:Background and objective The plasminogen (PLG) activation system plays an essential role in severe inflammation based diseases such as periodontitis, destructive membranous periodontal disease (ligneous periodontitis), familial Mediterranean fever (FMF), and amyloidosis. We have aimed to evaluate variations in PLG and the associations between PLG and MEFV genotypes in patients with FMF/ FMF‐related secondary amyloidosis and periodontitis. Material and methods A total of 247 individuals who were either diagnosed with FMF or systemically healthy were recruited to this human observational study with a cross‐sectional design. All individuals were also diagnosed with periodontitis or periodontally healthy. Blood samples were obtained from patients with FMF and systemically healthy controls. Clinical periodontal indicators were recorded. All polymorphisms located in exons 6 and 8 of PLG and mutations located on exons 2 and 10 of the MEFV gene were analyzed by DNA Sanger Sequencing. Genotypes and allele frequencies of PLG and MEFV were detected and tested by the Hardy‐Weinberg equilibrium. Serum levels of amyloid A (SAA), high‐sensitive C‐reactive protein (hs‐CRP), PLG, and salivary PLG levels were determined by using enzyme‐linked immunosorbent assay (ELISA). Results Two polymorphisms were identified in PLG: G to A polymorphism on the 14th nucleotide of intron 8 and C to T polymorphism on the 924th nucleotide of the coding region (IVS 8+14 G>A and c.924C>T, respectively). In IVS 8+14 G>A polymorphisms, wild‐type genotype: GG, heterozygote genotype: GA and homozygote genotype: AA. In c.924C>T polymorphism, wild‐type genotype: CC, heterozygote genotype: CT and homozygote genotype: TT. The frequency of the heterozygous polymorphisms of PLG was significantly increased (17.6%) in FMF patients with periodontitis (p = .027). A large proportion of the test group that was heterozygous for MEFV‐R202Q also had heterozygous PLG polymorphisms. Remarkable exacerbation in periodontal parameters was observed in patients with FMF and amyloidosis. SAA and hs‐CRP levels were significantly correlated with salivary PLG levels in patients with periodontitis and heterozygous PLG. Conclusions The current study describes IVS 8+14 G>A (rs2295368) and c.924C>T (rs1380916375) polymorphisms for the first time in the periodontal literature, which might play an important role in the pathogenesis of periodontitis, FMF, or amyloidosis. The elucidation of PLG polymorphisms is beneficial from a p
ISSN:0022-3484
1600-0765
DOI:10.1111/jre.12966