The novel homologue of the human α‐glucosidase inhibited by the non‐germinated and germinated quinoa protein hydrolysates after in vitro gastrointestinal digestion

Quinoa (Chenopodium quinoa Willd) is a potential source of protein with ideal amino acid profiles which its bioactive compounds can be improved during germination and gastrointestinal digestion. The present investigation studies the impact of germination for 24 hr and simulated gastrointestinal dige...

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Published in:Journal of food biochemistry 2022-01, Vol.46 (1), p.e14030-n/a
Main Authors: Salami, Maryam, Sadeghian Motahar, Seyedeh Fatemeh, Ariaeenejad, Shohreh, Sheykh Abdollahzadeh Mamaghani, Atefeh, Kavousi, Kaveh, Moosavi‐Movahedi, Ali A., Hosseini Salekdeh, Ghasem
Format: Article
Language:English
Subjects:
alpha-Glucosidases - metabolism
anti‐diabetic
bioactive peptide
Chenopodium quinoa - chemistry
Chenopodium quinoa - metabolism
Digestion
germination
Humans
Kinetics
metagenome
Protein Hydrolysates
quinoa protein
α‐glucosidase
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Summary:Quinoa (Chenopodium quinoa Willd) is a potential source of protein with ideal amino acid profiles which its bioactive compounds can be improved during germination and gastrointestinal digestion. The present investigation studies the impact of germination for 24 hr and simulated gastrointestinal digestion on α‐glucosidase inhibitory activity of the quinoa protein and bioactive peptides against the novel homologue of human α‐glucosidase, PersiAlpha‐GL1. The sprouted quinoa after gastroduodenal digestion was the most effective α‐glucosidase inhibitor showing 81.10% α‐glucosidase inhibition at concentration 4 mg/ml with the half inhibition rate (IC50) of 0.07 mg/ml. Based on the kinetic analysis, both the germinated and non‐germinated samples before and after digestion were competitive‐type inhibitors of α‐glucosidase. Results of this study showed the improved α‐glucosidase inhibitory activity of the quinoa bioactive peptides after germination and gastrointestinal digestion and highlighted the potential of metagenome‐derived PersiAlpha‐GL1 as a novel homologue of the human α‐glucosidase for developing the future anti‐diabetic drugs. Practical applications This study aimed to evaluate the effect of germination and gastrointestinal digestion of the quinoa protein and bioactive peptides on α‐glucosidase inhibitory activity against the novel PersiAlpha‐GL1. Metagenomic data were used to identify the novel α‐glucosidase structurally and functionally homologue of human intestinal. The results showed the highest inhibition on PersiAlpha‐GL1 by a germinated quinoa after gastroduodenal digestion and confirmed the potential of PersiAlpha‐GL1 to enhance the effectiveness of the anti‐diabetic drugs for industrial application. This study investigated the α‐glucosidase inhibitory effects of the quinoa protein and peptides after germination and gastrointestinal digestion against the novel homologue of human α‐glucosidase (PersiAlpha‐GL1). The metagenomic data was used to identify the novel α‐glucosidase. The germination and gastrointestinal digestion had a positive impact on the α‐glucosidase inhibitory activities. Kinetic study showed competitive‐type inhibition for both germinated and non‐germinated quinoa before and after digestion. Results confirmed the potential of metagenome‐derived PersiAlpha‐GL1 for the development of future anti‐diabetic drugs.
ISSN:0145-8884
1745-4514
DOI:10.1111/jfbc.14030