The moso bamboo WRKY transcription factor, PheWRKY86, regulates drought tolerance in transgenic plants

PheWRKY86 is a member of the WRKY transcription factor family in moso bamboo (Phyllostachys edulis). Expression of PheWRKY86 is strongly induced by drought and abscisic acid (ABA) treatments. The PheWRKY86 protein localizes to the cell nucleus and is specifically able to bind to W-box elements. 35S:...

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Bibliographic Details
Published in:Plant physiology and biochemistry 2022-01, Vol.170, p.180-191
Main Authors: Wu, Min, Zhang, Kaimei, Xu, Yuzeng, Wang, Linna, Liu, Hongxia, Qin, Zilu, Xiang, Yan
Format: Article
Language:English
Subjects:
ABA
Abscisic Acid
Drought
Droughts
Gene Expression Regulation, Plant
Moso bamboo
NCED1
PheWRKY86
Plant Proteins - genetics
Plant Proteins - metabolism
Plants, Genetically Modified - genetics
Plants, Genetically Modified - metabolism
Stress, Physiological - genetics
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:PheWRKY86 is a member of the WRKY transcription factor family in moso bamboo (Phyllostachys edulis). Expression of PheWRKY86 is strongly induced by drought and abscisic acid (ABA) treatments. The PheWRKY86 protein localizes to the cell nucleus and is specifically able to bind to W-box elements. 35S:PheWRKY86 transgenic Arabidopsis and rice showed significantly improved tolerance to drought stress. 35S:PheWRKY86 transgenic plants exhibited better water retention and lower relative electrolyte leakage (REL) and malondialdehyde (MDA) compared to wild type plants. Moreover, 35S:PheWRKY86 transgenic lines showed higher sensitivity to ABA stress. The 35S:PheWRKY86 transgenic plants exhibited higher ABA levels relative to wild type, while also exhibiting a lower germination rate, root length and fresh weight compared to wild type. Further analysis showed that expression of some ABA-responsive genes was changed in the 35S:PheWRKY86 transgenic lines under drought conditions. Transient expression and yeast one-hybrid assays demonstrated that PheWRKY86 could bind to the W-box element in the promoter region of NCED1. Taken together, these results demonstrate that PheWRKY86 plays a positive role in drought tolerance by regulating NCED1 expression. •PheWRKY86 is a member of the WRKY transcription factor family in moso bamboo (Phyllostachys edulis).•PheWRKY86 overexpression improves drought stress tolerance in transgenic plants.•Sensitivity of 35S:PheWRKY86 transgenic plants to exogenous ABA.•PheWRKY86 directly interacts with the promoter of OsNCED1.
ISSN:0981-9428
1873-2690
DOI:10.1016/j.plaphy.2021.10.024