Effect of Surface Layer Proteins Derived from Paraprobiotic Kefir Lactic Acid Bacteria on Inflammation and High-Fat Diet-Induced Obesity

The antiobesity action of nonviable probiotic lactic acid bacteria (PLAB) may be attributed to bacterial cellular components recognized by host cells. The anti-inflammation and antiobesity properties of surface layer proteins (SLPs) that are cellular components isolated from kefir PLAB were determin...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2021-12, Vol.69 (50), p.15157-15164
Main Authors: Kim, Eseul, Lee, Hyeon Gyu, Han, Sanghoon, Seo, Kun-Ho, Kim, Hyunsook
Format: Article
Language:English
Subjects:
Adipose Tissue
Animals
Bioactive Constituents, Metabolites, and Functions
Diet, High-Fat - adverse effects
Inflammation - genetics
Kefir
Lactobacillales - genetics
Membrane Glycoproteins
Mice
Mice, Inbred C57BL
Obesity - genetics
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Summary:The antiobesity action of nonviable probiotic lactic acid bacteria (PLAB) may be attributed to bacterial cellular components recognized by host cells. The anti-inflammation and antiobesity properties of surface layer proteins (SLPs) that are cellular components isolated from kefir PLAB were determined in macrophage RAW 264.7 cells and obese mice. Kefir SLPs significantly decreased secretion of IL-6 and production of NF-kB p65 protein by LPS-stimulated RAW 264.7 cells in a dose–response manner. C57BL/6J mice were fed a high-fat (HF) diet with oral administration of either saline (CON) or kefir SLPs for 6 weeks. SLPs significantly improved body weight gain and adipose tissue weight, plasma triglyceride concentrations, and insulin resistance. Profiling of adipocyte gene expression showed that the antiobesity effect was significantly related to the expression of genes associated with adipogenesis, autophagy, and inflammatory/immune response, and fatty acid oxidation. Taken together, SLPs are a novel bioactive component in kefir PLABs to target obesity and obesity-related disorders.
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.1c05037