L‐carnitine added to post‐thawed semen acts as an antioxidant and a stimulator of equine sperm metabolism

The objective of this study was to enhance the in vitro sperm quality and in vivo fertility of frozen‐thawed equine semen by the addition of l‐carnitine (LC) to post‐thawed semen. Different concentrations of LC were added to thawed samples to obtain four treatments control and 0.5, 1 and 2 mM LC. In...

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Published in:Andrologia 2022-04, Vol.54 (3), p.e14338-n/a
Main Authors: Lagares, Monique de Albuquerque, Silva, Grazielle Caroline, Cortes, Steyner Franca, Moreira, Fabio Henrique Marques, Neves, Franciele Caroline Dias, Alves, Natália de Castro, Viegas, Rodrigo Novaes, Diniz, Thiago Frederico, Lemos, Virginia Soares, Rezende, Adalgiza Souza Carneiro, Freitas, Marina Morra, Stahlberg, Rubens, Nicolino, Rafael Romero
Format: Article
Language:English
Subjects:
Animals
Antioxidants
Antioxidants - pharmacology
Artificial insemination
Biological activity
Calcium (intracellular)
Carnitine
Carnitine - pharmacology
cryopreservation
Cryopreservation - veterinary
Female
Fertility
frozen semen
Horses
Hydrogen peroxide
Insemination, Artificial - veterinary
intracellular calcium
Kinematics
L-Carnitine
Male
Metabolism
nitrite
Nitrogen dioxide
Pregnancy
Semen
Semen Preservation - veterinary
Sperm
Sperm Motility
Spermatozoa
stallion
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Summary:The objective of this study was to enhance the in vitro sperm quality and in vivo fertility of frozen‐thawed equine semen by the addition of l‐carnitine (LC) to post‐thawed semen. Different concentrations of LC were added to thawed samples to obtain four treatments control and 0.5, 1 and 2 mM LC. In the in vitro experiments, sperm motility and kinematics, membrane integrity and intracellular calcium ion concentration ([Ca2+]i) were investigated, and the antioxidant bioactivity of LC was assessed by measuring hydrogen peroxide and nitrite concentrations (NO2 −). The fertility rate was assessed via the artificial insemination of mares. The treatment with 1 mM LC increased sperm [Ca2+]i (60.6 ± 0.05 AU), reduced nitrite concentration (39.1 ± 14.9 µM/µg protein), increased the sperm straightness percentage (STR: 78.3 ± 5.3%) and increased the pregnancy rate (75%) as compared to the control ([Ca2+]i 48.4 ± 0.05 AU, NO2 − concentration 63.1 ± 14.4 µM/µg protein, STR 67.5 ± 7.9%, 12.5% pregnancy rate, p 
ISSN:0303-4569
1439-0272
DOI:10.1111/and.14338