Self-Terminated Electroless Deposition of Surfactant-Free and Monodispersed Pt Nanoparticles on Carbon Fiber Microelectrodes for Sensitive Detection of H2O2 Released from Living Cells

We report a self-terminated electroless deposition method to prepare surfactant-free and monodispersed Pt nanoparticle (NP)-modified carbon fiber microelectrodes (Pt NP/CFEs) for electrochemical detection of hydrogen peroxide (H2O2) released from living cells. The surfactant-free and monodispersed P...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2021-12, Vol.93 (49), p.16683-16689
Main Authors: Tong, Yuxi, Wang, Lifen, Song, Jiajia, Zhang, Mengyue, Qi, Hetong, Ding, Shujiang, Qi, Honglan
Format: Article
Language:English
Subjects:
Carbon
Carbon fibers
Cells (biology)
Chemistry
Electrochemical analysis
Electrochemistry
Electrodes
Electroless deposition
Electroless plating
Fabrication
Gold
Hydrogen peroxide
Metal ions
Microelectrodes
Nanoparticles
Oxidation
Platinum
Reaction time
Reducing agents
Reduction (metal working)
Reproducibility
Sensitivity
Silver
Spatial discrimination
Spatial resolution
Surfactants
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Summary:We report a self-terminated electroless deposition method to prepare surfactant-free and monodispersed Pt nanoparticle (NP)-modified carbon fiber microelectrodes (Pt NP/CFEs) for electrochemical detection of hydrogen peroxide (H2O2) released from living cells. The surfactant-free and monodispersed Pt NPs with a uniform size of 65 nm are spontaneously deposited on a CFE surface by immersing an exposed carbon fiber (CF) of CFE in the PtCl4 2– solution, in which an exposed CF can be used as the reducing agent and stabilizer. A self-terminated electroless deposition method is demonstrated, in which the density and size of Pt NPs on a CFE surface do not increase when the reaction time increases from 20 to 60 min. The self-terminated electroless deposition process not only can effectively avoid any manual electrode modification and thus largely minimize person-to-person and electrode-to-electrode deviations but also can avoid the use of any extra reductant or surfactant in the fabrication process. Therefore, Pt NPs/CFEs, with good reproducibility and sensitivity, not only exhibit high electrocatalytic activity toward the oxidation of H2O2 but also maintain the spatial resolution of CFEs. Moreover, Pt NPs/CFEs can detect H2O2 with a wide linear range of 0.5–80 μM and a low detection limit of 0.17 μM and then can be successfully applied in the monitoring of H2O2 released from RAW 264.7 cells. The self-terminated electroless deposition method can also be extended to selectively prepare other metal NP-modified CFEs, such as Au NPs/CFEs or Ag NPs/CFEs, by choosing the metal ions with higher reduction potential as precursors. This work provides a simple, straightforward, and general method for the preparation of small, surfactant-free, and monodispersed metal NP-modified CFEs with high sensitivity, reproducibility, and spatial resolution.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.1c04299