Differentiated embryo chondrocyte 1, induced by hypoxia-inducible factor 1α, promotes cell migration in oral squamous cell carcinoma cell lines

This study aimed to explore the correlation between differentiated embryo chondrocyte 1 (DEC1) and hypoxia-inducible factor 1α (HIF-1α) in oral squamous cell carcinoma (OSCC) and how they participate in tumor progression. An immunohistochemical staining method was used to detect the expression of HI...

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Bibliographic Details
Published in:Oral surgery, oral medicine, oral pathology and oral radiology oral medicine, oral pathology and oral radiology, 2022-02, Vol.133 (2), p.199-206
Main Authors: Yang, Liudi, Zeng, Liujun, Wang, Zijia, Hu, Xin, Xiong, Haofeng, Zhang, Tianyi, Chen, Weijun, Xia, Kun, Su, Tong
Format: Article
Language:English
Subjects:
Cell Hypoxia - genetics
Cell Line, Tumor
Cell Movement
Chondrocytes
Humans
Hypoxia
Hypoxia-Inducible Factor 1, alpha Subunit - metabolism
Mouth Neoplasms - pathology
Squamous Cell Carcinoma of Head and Neck - pathology
Tumor Suppressor Proteins - metabolism
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Summary:This study aimed to explore the correlation between differentiated embryo chondrocyte 1 (DEC1) and hypoxia-inducible factor 1α (HIF-1α) in oral squamous cell carcinoma (OSCC) and how they participate in tumor progression. An immunohistochemical staining method was used to detect the expression of HIF-1α and DEC1 in 64 OSCC specimens, and the correlation between HIF-1α and DEC1 was analyzed. The expression of HIF-1α and DEC1 in OSCC cells under normoxic and hypoxic environments was assessed and analyzed by Western blotting and immunofluorescence. Furthermore, the DEC1 gene was silenced by siRNA and treated with cobalt chloride (CoCl2) to analyze the effects that DEC1 and hypoxia might have on the migration ability of OSCC cells. The expression of HIF-1α and DEC1 in OSCC was positively correlated. Using CoCl2 to simulate a hypoxic environment increased the protein levels of HIF-1α and DEC1 in OSCC cells. The HIF-1α inhibitor LW6 decreased HIF-1α and DEC1 expression in OSCC cells in a hypoxic environment. Silencing the DEC1 gene reduced the migration ability of OSCC cells. The hypoxic environment in OSCC could upregulate the expression of DEC1 by increasing the protein level of HIF-1α, and this process might be involved in the migration of tumor cells.
ISSN:2212-4403
2212-4411
DOI:10.1016/j.oooo.2021.08.022