Interactions of E. coli with cylindrical micro-pillars of different geometric modifications

Understanding the behavior of bacteria at the proximity of different surfaces is of great importance and interest. Despite recent exciting progress in geometric control of bacterial behavior around surfaces, a detailed comparison on the interaction of bacteria with cylindrical surfaces of different...

Full description

Saved in:
Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2022-01, Vol.209 (Pt 2), p.112190-112190, Article 112190
Main Authors: Krishnamurthi, Venkata Rao, Harris, Nathaniel, Rogers, Ariel, Zou, Min, Wang, Yong
Format: Article
Language:English
Subjects:
3D printing
Escherichia coli
geometry
microstructures
surface
two-photon polymerization
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Understanding the behavior of bacteria at the proximity of different surfaces is of great importance and interest. Despite recent exciting progress in geometric control of bacterial behavior around surfaces, a detailed comparison on the interaction of bacteria with cylindrical surfaces of different geometric modifications is still missing. Here, we investigated how bacteria interacted with cylindrical micro-pillars and modified cylindrical micro-pillars with sprocket, gear, and flower-like wall surface features. Using phase-contrast microscopy, we examined the motion of bacteria around the micro-pillars, and observed different responses of bacteria to each geometric modification. In addition, we extracted the trajectories of the bacteria and characterized several parameters (instantaneous velocity v, change of direction δ, approaching angle ϕ) to quantitatively compare the effects of the geometric modifications on the micro-pillars. We found that sharp spikes showed the largest effect, compared to smooth surface, convex and concave ripples. Lastly, we carried out numerical simulations, which explained the experimental observations and showed that the observed effects were due to the geometric modifications. [Display omitted] •Bacterial behavior near cylindrical micro-pillars is quantified and compared.•Bacteria respond differently to sprocket, gear, and flower-like modifications.•Gear-like micro-pillars slow down and trap bacteria.•Gear-like micro-pillars change the run-and-tumble dynamics of bacteria.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2021.112190