Therapeutic potential of mesenchymal stem cells for scleroderma induced in mouse model

•Scleroderma is a connective tissue disorder characterized by activated fibroblasts and altered dermal cytokine levels.•Level of NO, α-SMA and TGF-β1 significantly normalized after MSC therapy over the 7 weeks duration of the study.•Mesenchymal stem cells therapy is a potentially promising therapeut...

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Published in:Tissue & cell 2021-12, Vol.73, p.101671-101671, Article 101671
Main Authors: Elessawi, Dina Fathy, Gabr, Hala, Badawy, Monda Mohamed Maher, Gheita, Tamer A.
Format: Article
Language:English
Subjects:
Actin
Animal tissues
Animals
Disease Models, Animal
Growth factors
Histopathology
Humans
Hypochlorous acid
Intravenous administration
Lungs
Mesenchymal Stem Cell Transplantation
Mesenchymal stem cells
Mesenchymal Stem Cells - cytology
Mice
Mouse model
Muscles
Nitric oxide
Nitric Oxide - blood
Parenchyma
Scleroderma
Scleroderma, Systemic - blood
Scleroderma, Systemic - therapy
Skin
Skin - pathology
Smooth muscle
Stem cell transplantation
Stem cells
Therapy
Tissue analysis
Transforming Growth Factor beta - metabolism
Transforming growth factor-b
Transforming growth factor-b1
Transforming growth factor-β
α-smooth muscle actin
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Summary:•Scleroderma is a connective tissue disorder characterized by activated fibroblasts and altered dermal cytokine levels.•Level of NO, α-SMA and TGF-β1 significantly normalized after MSC therapy over the 7 weeks duration of the study.•Mesenchymal stem cells therapy is a potentially promising therapeutic option for systemic sclerosis. To examine the potential therapeutic effect of mesenchymal stem cells (MSCs) for experimental scleroderma. Fifty-four mice six-week-old (30−35 g) were studied. Hypochlorous acid (HOCl) induced scleroderma was considered. Mice were divided into 3 groups: (I) Control: Six mice did not receive any treatment and were sacrificed at the end of the experiment; (II) HOCl mice (induced scleroderma as a positive control): (III) MSCs-treated HOCl mice: Thirty six HOCl-induced mice were injected with MSCs (7.5 × 105) intravenous every week for 3 weeks. Skin pieces were taken from the backs of mice and lung tissue pieces. a smooth muscle actin (α-SMA) and transforming growth factor-β (TGF-β1) were analysed or fixed in 10 % formalin for skin and lung tissue histopathological analysis. Plasma nitric oxide (NO) was also assayed. There was a significant rise in the NO level and of the cutaneous and lung tissue α-SMA and TGF-β1 in untreated scleroderma-induced mice. The values significantly normalized after MSC therapy over the 7 weeks duration of the study. The altered histopathology of the skin and lung tissues in the scleroderma-induced mice showed a remarkable tendency to normalization of the skin and lung parenchyma and vasculature. There was a significant rise in the level of NO and skin and lung tissue α-SMA and TGF-β1 in untreated scleroderma-induced mice and values were significantly normalized after MSC therapy over the 7 weeks duration of the study. Altered histopathology of the skin and lung appeared nearly normal after MSC therapy.
ISSN:0040-8166
1532-3072
DOI:10.1016/j.tice.2021.101671