Comprehensive proteomic analysis of sea cucumbers (Stichopus japonicus) in thermal processing by HPLC-MS/MS

•The total protein loss of sea cucumber in high pressure steaming was the lowest.•Proteins undergoes unspecific hydrolysis during the thermal processing.•The protein residues with the highest thermal-induced hydrolysis frequency were Phe, Leu, Asn, and Tyr. Thermal processing is the most frequently...

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Bibliographic Details
Published in:Food chemistry 2022-03, Vol.373 (Pt A), p.131368-131368, Article 131368
Main Authors: Gu, Pan, Qi, Shizhe, Zhai, Ziyang, Liu, Jing, Liu, Zheyi, Jin, Yan, Qi, Yanxia, Zhao, Qiancheng, Wang, Fangjun
Format: Article
Language:English
Subjects:
Animals
Chromatography, High Pressure Liquid
Hydrolysis site
Mass spectrometry
Proteomic analysis
Proteomics
Sea Cucumbers
Stichopus
Stichopus japonicus
Tandem Mass Spectrometry
Thermal processing
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Summary:•The total protein loss of sea cucumber in high pressure steaming was the lowest.•Proteins undergoes unspecific hydrolysis during the thermal processing.•The protein residues with the highest thermal-induced hydrolysis frequency were Phe, Leu, Asn, and Tyr. Thermal processing is the most frequently adopted processing technology for sea cucumbers, which can significantly affect their protein composition. In this paper, three thermal processing methods high pressure steaming (HPS), atmospheric pressure boiling (APB), and atmospheric pressure steaming (APS) were adopted and protein compositions of both body walls and cooking liquors by thermal processing stichopus japonicus were systematically analysis by proteomic strategy. The total proteins loss rates of body walls were 11.6%, 13.0%, and 14.8% for HPS, APS, and APB methods, respectively. However, the main types of protein composition were retained. Similar mechanisms of protein loss may exist even if different thermal processing were applied. The most frequent hydrolysis sites in thermal processing were phenylalanine, leucine, asparagine, and tyrosine at both C and N terminals. This study provides theoretical guidance for optimizing the industry thermal processing of sea cucumbers.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2021.131368