Elicitation of defense response by transglycosylated chitooligosaccharides in rice seedlings

Long-chain chitooligosaccharides (COS) with degree of polymerization (DP) more than 4 are known to have potential biological activities. A hyper-transglycosylating mutant of an endo-chitinase from Serratia proteamaculans (SpChiD-Y28A) was used to synthesize COS with DP6 and DP7 using COS DP5 as subs...

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Bibliographic Details
Published in:Carbohydrate research 2021-12, Vol.510, p.108459-108459, Article 108459
Main Authors: Ramakrishna, Bellamkonda, Sarma, P.V.S.R.N., Ankati, Sravani, Bhuvanachandra, Bhoopal, Podile, Appa Rao
Format: Article
Language:English
Subjects:
Chitinase
Chitooligosaccharides
Chitosan - chemistry
Chitosan - metabolism
Defense mechanism
Elicitor activity
Glycosylation
Oligosaccharides - chemistry
Oligosaccharides - metabolism
Oryza - chemistry
Oryza - metabolism
Seedlings - chemistry
Seedlings - metabolism
Serratia - chemistry
Serratia - metabolism
Transglycosylation
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Summary:Long-chain chitooligosaccharides (COS) with degree of polymerization (DP) more than 4 are known to have potential biological activities. A hyper-transglycosylating mutant of an endo-chitinase from Serratia proteamaculans (SpChiD-Y28A) was used to synthesize COS with DP6 and DP7 using COS DP5 as substrate. Purified COS with DP5-7 were tested to elicit the defense response in rice seedlings. Among the COS used, DP7 strongly induced oxidative burst response as well as peroxidase, and phenylalanine ammonia lyase activites. A few selected marker genes in salicylic acid (SA)- and jasmonic acid-dependent pathways were evaluated by real-time PCR. The expression levels of pathogenesis-related (PR) genes PR1a and PR10 and defense response genes (chitinase1, peroxidase and β -1,3-glucanase) were up regulated upon COS treatment in rice seedlings. The DP7 induced Phenylalanine ammonia lyase and Isochorismate synthase 1 genes, with concomitant increase of Mitogen-activated protein kinase 6 and WRKY45 transcription factor genes indicated the possible role of phosphorylation in the transmission of a signal to induce SA-mediated defense response in rice. [Display omitted] •Foliar treatment with COS elicited rapid and longer oxidative burst response in rice.•COS with DP7 exhibited higher induction of defense-related genes than DP6 and DP5.•COS triggered SA signaling pathway genes for defense in rice over JA pathway.•PR1a, PR10, chitinase1, peroxidase and β -1,3-glucanase genes were induced by DP7.•Induction of MAPK6a and WRKY45 genes was enhanced with DP7 treatment.
ISSN:0008-6215
1873-426X
DOI:10.1016/j.carres.2021.108459