How long does the mRNA remains stable in untreated whole bovine blood?

Background High quality and quantity of messenger RNA (mRNA) are required for accuracy of gene expression studies and other RNA-based downstream applications. Since RNA is considered a labile macromolecular prone to degradation, which may result in falsely altered gene expression patterns, several c...

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Bibliographic Details
Published in:Molecular biology reports 2022, Vol.49 (1), p.789-795
Main Authors: Giglioti, Rodrigo, Azevedo, Bianca Tainá, de Oliveira, Henrique Nunes, Katiki, Luciana Morita, Filho, Anibal Eugênio Vercesi, de Sena Oliveira, Márcia Cristina, Okino, Cintia Hiromi
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Biomedical and Life Sciences
CD4 antigen
Gene expression
Glyceraldehyde-3-phosphate dehydrogenase
Histology
Life Sciences
Macromolecules
Morphology
mRNA stability
Sampling
Short Communication
Transcription
γ-Interferon
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Summary:Background High quality and quantity of messenger RNA (mRNA) are required for accuracy of gene expression studies and other RNA-based downstream applications. Since RNA is considered a labile macromolecular prone to degradation, which may result in falsely altered gene expression patterns, several commercial stabilizing reagents have been developed aiming to keep RNA stable for long period. However, for studies involving large number of experimental samples, the high costs related to these specific reagents may constitute a barrier. Methods and results In this context the present study was designed aiming to evaluate the stability of mRNA in whole bovine blood collected in EDTA tubes during storage at common fridge (4 °C). Whole blood samples were collected from six Holstein calves and submitted to RNA extraction in each different interval: immediately after blood sampling (
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-021-06808-w